TY - JOUR
T1 - Indian gooseberry (Emblica officinalis Gaertn.) suppresses cell proliferation and induces apoptosis in human colon cancer stem cells independent of p53 status via suppression of c-Myc and cyclin D1
AU - Vadde, Ramakrishna
AU - Radhakrishnan, Sridhar
AU - Eranda Karunathilake Kurundu, Hewage
AU - Reddivari, Lavanya
AU - Vanamala, Jairam K.P.
N1 - Funding Information:
We acknowledge Venkata R Charepalli and Aaron Massey for their help in laboratory assistance. We thank the Department of Biotechnology (DBT), Government of India, for awarding DBT CREST Fellowship/Award to Ramakrishna Vadde for travel and living expenses to conduct research in Dr. Vanamala's laboratory. We thank the Proteomics and Metabolomics Core Facility at Colorado State University and the Metabolomics Core Facility at Penn State University for assistance with LC-MS/MS. This work was supported by the National Research Initiative Grant 2009-55200-05197 from the USDA National Institute for Food and Agriculture to Jairam KP Vanamala.
Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Indian gooseberry, also known as amla, a widely consumed fruit in South Asia, was evaluated for its anti-proliferative and pro-apoptotic mechanisms on human colon cancer stem cells (HCCSC). Amla extracts suppressed proliferation and induced apoptosis independent of p53, a tumour suppressor gene, in HCCSCs. Further, amla extracts suppressed cell proliferation by targeting the Wnt/β-catenin signalling pathway as seen by decreased nuclear translocation of β-catenin. Additionally, this led to suppressed expression of c-Myc and cyclin D1, key proteins involved in cell proliferation. Inhibition of stem-ness of HCCSCs by amla may be due to its effect on the Wnt/β-catenin signalling. These results indicate that amla suppresses HCCSC proliferation and induces apoptosis independent of p53 status via potentially targeting Wnt/β-catenin signalling pathway. Amla is therefore a promising functional food for preventing colon cancer and might be a novel resource for the food industry.
AB - Indian gooseberry, also known as amla, a widely consumed fruit in South Asia, was evaluated for its anti-proliferative and pro-apoptotic mechanisms on human colon cancer stem cells (HCCSC). Amla extracts suppressed proliferation and induced apoptosis independent of p53, a tumour suppressor gene, in HCCSCs. Further, amla extracts suppressed cell proliferation by targeting the Wnt/β-catenin signalling pathway as seen by decreased nuclear translocation of β-catenin. Additionally, this led to suppressed expression of c-Myc and cyclin D1, key proteins involved in cell proliferation. Inhibition of stem-ness of HCCSCs by amla may be due to its effect on the Wnt/β-catenin signalling. These results indicate that amla suppresses HCCSC proliferation and induces apoptosis independent of p53 status via potentially targeting Wnt/β-catenin signalling pathway. Amla is therefore a promising functional food for preventing colon cancer and might be a novel resource for the food industry.
UR - http://www.scopus.com/inward/record.url?scp=84975252027&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975252027&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2016.06.007
DO - 10.1016/j.jff.2016.06.007
M3 - Article
AN - SCOPUS:84975252027
SN - 1756-4646
VL - 25
SP - 267
EP - 278
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -
