Indirect interspecies regulation: Transcriptional and physiological responses of a cyanobacterium to heterotrophic partnership

Hans C. Bernstein, Ryan S. McClure, Vera Thiel, Natalie C. Sadler, Young Mo Kim, William B. Chrisler, Eric A. Hill, Donald A. Bryant, Margaret F. Romine, Janet K. Jansson, Jim K. Fredrickson, Alexander S. Beliaev

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The mechanisms by which microbes interact in communities remain poorly understood. Here, we interrogated specific interactions between photoautotrophic and heterotrophic members of a model consortium to infer mechanisms that mediate metabolic coupling and acclimation to partnership. This binary consortium was composed of a cyanobacterium, Thermosynechococcus elongatus BP-1, which supported growth of an obligate aerobic heterotroph, Meiothermus ruber strain A, by providing organic carbon, O2, and reduced nitrogen. Species-resolved transcriptomic analyses were used in combination with growth and photosynthesis kinetics to infer interactions and the environmental context under which they occur. We found that the efficiency of biomass production and resistance to stress induced by high levels of dissolved O2 increased, beyond axenic performance, as a result of heterotrophic partnership. Coordinated transcriptional responses transcending both species were observed and used to infer specific interactions resulting from the synthesis and exchange of resources. The cyanobacterium responded to heterotrophic partnership by altering expression of core genes involved with photosynthesis, carbon uptake/fixation, vitamin synthesis, and scavenging of reactive oxygen species (ROS).

Original languageEnglish (US)
Article numbere00181
JournalmSystems
Volume2
Issue number2
DOIs
StatePublished - Mar 1 2017

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Physiology
  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Molecular Biology
  • Genetics
  • Computer Science Applications

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