Induced gene transcription

Implications for biomarkers

C. H. Sewall, D. A. Bell, G. C. Clark, A. M. Tritscher, D. B. Tully, John Patrick Vanden Heuvel, G. W. Lucier

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Numerous xenobiotics regulate cellular functions by altering transcription of target genes. Use of sensitive and specific biomarkers based on gene transcript levels may help clarify the shape of the dose-response curve in the low-dose region associated with human exposures to environmental concentrations of chemicals. We have quantified gene transcription induced by 2,3,7,8-tetra-chlorodibenzo-p-dioxin in both animal models and humans with the use of Northern analysis and PCR-based methods. In addition, we describe a rapid and sensitive in vitro assay that we have used to screen chemicals and biological samples for their ability to alter gene transcription. Whereas some of the endpoints in our studies such as cytochrome P-450 1A1 are predictive indicators of exposure and dose, other gene responses such as growth factors are more complex and represent a critical event, progression, or adaptation to a pathological alteration. In conclusion, measurement of toxicant-induced gene transcription will contribute to the usefulness of biomarkers in addressing issues of human health and environmentally induced disease.

Original languageEnglish (US)
Pages (from-to)1829-1834
Number of pages6
JournalClinical Chemistry
Volume41
Issue number12 SUPPL.
StatePublished - Jan 1 1995

Fingerprint

Biomarkers
Transcription
Genes
Environmental Exposure
Xenobiotics
Cytochrome P-450 Enzyme System
Assays
Intercellular Signaling Peptides and Proteins
Animals
Animal Models
Health
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Sewall, C. H., Bell, D. A., Clark, G. C., Tritscher, A. M., Tully, D. B., Vanden Heuvel, J. P., & Lucier, G. W. (1995). Induced gene transcription: Implications for biomarkers. Clinical Chemistry, 41(12 SUPPL.), 1829-1834.
Sewall, C. H. ; Bell, D. A. ; Clark, G. C. ; Tritscher, A. M. ; Tully, D. B. ; Vanden Heuvel, John Patrick ; Lucier, G. W. / Induced gene transcription : Implications for biomarkers. In: Clinical Chemistry. 1995 ; Vol. 41, No. 12 SUPPL. pp. 1829-1834.
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Sewall, CH, Bell, DA, Clark, GC, Tritscher, AM, Tully, DB, Vanden Heuvel, JP & Lucier, GW 1995, 'Induced gene transcription: Implications for biomarkers', Clinical Chemistry, vol. 41, no. 12 SUPPL., pp. 1829-1834.

Induced gene transcription : Implications for biomarkers. / Sewall, C. H.; Bell, D. A.; Clark, G. C.; Tritscher, A. M.; Tully, D. B.; Vanden Heuvel, John Patrick; Lucier, G. W.

In: Clinical Chemistry, Vol. 41, No. 12 SUPPL., 01.01.1995, p. 1829-1834.

Research output: Contribution to journalArticle

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AU - Vanden Heuvel, John Patrick

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Sewall CH, Bell DA, Clark GC, Tritscher AM, Tully DB, Vanden Heuvel JP et al. Induced gene transcription: Implications for biomarkers. Clinical Chemistry. 1995 Jan 1;41(12 SUPPL.):1829-1834.