Inducibility of various cytochrome p450 isozymes by phenobarbital and some other xenobiotics in drosophila melanogaster

Serge Yu Fuchs, Vladimir Spiegelman, Gennady A. Belitsky

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The inducibility of cytochrome P450 isozymes has been investigated in the Drosophila melanogaster insecticide susceptible (Oregon R) and insecticide resistant (91R) strains. Both the level and induction kinetics of 7-ethoxycoumarine O-deethylase activity were stimulated by phenobarbital (PB) to a lower extent than that of aryl hydrocarbon hydroxylase in the Oregon R strain. The basal level of the cytochrome P450-linked activities in insecticide resistant flies was higher than that noted in susceptible ones. However, treatment with PB has increased levels of 7-ethoxycoumarine O-deethylase and aryl hydrocarbon hydroxylase activities more in susceptible flies than in resistant ones. in contrast to PB, the polycyclic aromatic hydrocarbon benzo[a]pyrene induced both activities in 91R flies to a greater extent than in Oregon R ones. The potent PB-like inducer in mice but not in rats 1.4-bis[2-(dichloropyridyloxy)]-benzene failed to induce the cytochrome P450 system in D. melanogaster, when triphenyldioxane (PB-like inducer in rats but not in mice) markedly affected this system in a PB-like manner. The SDS-PAGE followed by immunoblotting analysis using monoclonal antibodies 13-2e and 8-1d have shown that the level of the 56,000 and 54,000 Da insecticide resistance-related forms has increased in the susceptible strain by PB and some other PB like inducers. The relationship between these isozymes appearance and 7-ethoxycoumarine O-deethylase activity has been discussed.

Original languageEnglish (US)
Pages (from-to)1867-1873
Number of pages7
JournalBiochemical Pharmacology
Volume47
Issue number10
DOIs
StatePublished - May 18 1994

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Xenobiotics
Phenobarbital
Drosophila melanogaster
Cytochrome P-450 Enzyme System
Isoenzymes
Insecticides
Diptera
Aryl Hydrocarbon Hydroxylases
Rats
Insecticide Resistance
Benzo(a)pyrene
Polycyclic Aromatic Hydrocarbons
Benzene
Immunoblotting
Polyacrylamide Gel Electrophoresis
Monoclonal Antibodies
Kinetics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology

Cite this

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title = "Inducibility of various cytochrome p450 isozymes by phenobarbital and some other xenobiotics in drosophila melanogaster",
abstract = "The inducibility of cytochrome P450 isozymes has been investigated in the Drosophila melanogaster insecticide susceptible (Oregon R) and insecticide resistant (91R) strains. Both the level and induction kinetics of 7-ethoxycoumarine O-deethylase activity were stimulated by phenobarbital (PB) to a lower extent than that of aryl hydrocarbon hydroxylase in the Oregon R strain. The basal level of the cytochrome P450-linked activities in insecticide resistant flies was higher than that noted in susceptible ones. However, treatment with PB has increased levels of 7-ethoxycoumarine O-deethylase and aryl hydrocarbon hydroxylase activities more in susceptible flies than in resistant ones. in contrast to PB, the polycyclic aromatic hydrocarbon benzo[a]pyrene induced both activities in 91R flies to a greater extent than in Oregon R ones. The potent PB-like inducer in mice but not in rats 1.4-bis[2-(dichloropyridyloxy)]-benzene failed to induce the cytochrome P450 system in D. melanogaster, when triphenyldioxane (PB-like inducer in rats but not in mice) markedly affected this system in a PB-like manner. The SDS-PAGE followed by immunoblotting analysis using monoclonal antibodies 13-2e and 8-1d have shown that the level of the 56,000 and 54,000 Da insecticide resistance-related forms has increased in the susceptible strain by PB and some other PB like inducers. The relationship between these isozymes appearance and 7-ethoxycoumarine O-deethylase activity has been discussed.",
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Inducibility of various cytochrome p450 isozymes by phenobarbital and some other xenobiotics in drosophila melanogaster. / Fuchs, Serge Yu; Spiegelman, Vladimir; Belitsky, Gennady A.

In: Biochemical Pharmacology, Vol. 47, No. 10, 18.05.1994, p. 1867-1873.

Research output: Contribution to journalArticle

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