Induction and purification of c. Difficile phage tail-like particles

John P. Hegarty, William Sangster, Robert E. Ashley, Roland Myers, Susan Hafenstein, David B. Stewart

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Due to the inherent limitations of conventional antibiotics for the treatment of C. difficile infection (CDI), there is a growing interest in the development of alternative treatment strategies. Both bacteriophages and R-type bacteriocins, also known as phage tail-like particles (PTLPs), show promise as potential antibacterial alternatives for treating CDI. Similar to bacteriophages, but lacking a viral capsid and genome, PTLPs remain capable of killing target bacteria. Here we describe our experience in the induction and purification of C. difficile PTLPs. These methods have been optimized to allow production of concentrated, non- contractile, and non-aggregated samples for both sensitivity testing and structural electron microscopy studies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages167-175
Number of pages9
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1476
ISSN (Print)1064-3745

Fingerprint

Bacteriophages
Bacteriocins
Viral Genome
Capsid
Infection
Electron Microscopy
Anti-Bacterial Agents
Bacteria

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Hegarty, J. P., Sangster, W., Ashley, R. E., Myers, R., Hafenstein, S., & Stewart, D. B. (2016). Induction and purification of c. Difficile phage tail-like particles. In Methods in Molecular Biology (pp. 167-175). (Methods in Molecular Biology; Vol. 1476). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6361-4_12
Hegarty, John P. ; Sangster, William ; Ashley, Robert E. ; Myers, Roland ; Hafenstein, Susan ; Stewart, David B. / Induction and purification of c. Difficile phage tail-like particles. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 167-175 (Methods in Molecular Biology).
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Hegarty, JP, Sangster, W, Ashley, RE, Myers, R, Hafenstein, S & Stewart, DB 2016, Induction and purification of c. Difficile phage tail-like particles. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1476, Humana Press Inc., pp. 167-175. https://doi.org/10.1007/978-1-4939-6361-4_12

Induction and purification of c. Difficile phage tail-like particles. / Hegarty, John P.; Sangster, William; Ashley, Robert E.; Myers, Roland; Hafenstein, Susan; Stewart, David B.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 167-175 (Methods in Molecular Biology; Vol. 1476).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Hegarty JP, Sangster W, Ashley RE, Myers R, Hafenstein S, Stewart DB. Induction and purification of c. Difficile phage tail-like particles. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 167-175. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6361-4_12