Induction and variants of neuronal nitric oxide synthase type I during synaptogenesis

P. Ogilvie, K. Schilling, M. L. Billingsley, H. H.H.W. Schmidt

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93 Scopus citations

Abstract

In the adult central nervous system, nitric oxide (NO) is formed from L- arginine by the so-called constitutive or type I NO synthase (NOS-I155). However, expression of NOS-1155 immunoreactivity and activity was low or not detectable in developing mouse and rat brain. NOS-I155 was sharply induced coincident with the onset of synaptogenesis in specific brain regions. This was followed by a second phase in which total NOS-I155 expression decreased both in specific cell populations and in the total synaptosomal subcellular fraction. Furthermore, two putative variants of NOS-I were transiently observed: an NOS-I-immunoreactive protein with increased electrophoretic mobility (NOS-I144) and a transient hypersensitivity of NOS-I155 to the competitive substrate inhibitor N(ω)-nitro-L-arginine. It is concluded that NOS-I expression is not constitutive but locally induced. In the central nervous system, this regionally specific, bipbasic pattern of postnatal NOS-I induction is consistent with a role for NO in synaptogenesis and synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)799-806
Number of pages8
JournalFASEB Journal
Volume9
Issue number9
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Ogilvie, P., Schilling, K., Billingsley, M. L., & Schmidt, H. H. H. W. (1995). Induction and variants of neuronal nitric oxide synthase type I during synaptogenesis. FASEB Journal, 9(9), 799-806.