Induction of an antibody that catalyzes the hydrolysis of an amide bond

Kim D. Janda, Diane Schloeder, Stephen Benkovic, Richard A. Lerner

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

Original languageEnglish (US)
Pages (from-to)1188-1191
Number of pages4
JournalScience
Volume241
Issue number4870
DOIs
StatePublished - Jan 1 1988

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Amides
Hydrolysis
amidase
Antibodies
Catalysis

All Science Journal Classification (ASJC) codes

  • General

Cite this

Janda, K. D., Schloeder, D., Benkovic, S., & Lerner, R. A. (1988). Induction of an antibody that catalyzes the hydrolysis of an amide bond. Science, 241(4870), 1188-1191. https://doi.org/10.1126/science.3413482
Janda, Kim D. ; Schloeder, Diane ; Benkovic, Stephen ; Lerner, Richard A. / Induction of an antibody that catalyzes the hydrolysis of an amide bond. In: Science. 1988 ; Vol. 241, No. 4870. pp. 1188-1191.
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Janda, KD, Schloeder, D, Benkovic, S & Lerner, RA 1988, 'Induction of an antibody that catalyzes the hydrolysis of an amide bond', Science, vol. 241, no. 4870, pp. 1188-1191. https://doi.org/10.1126/science.3413482

Induction of an antibody that catalyzes the hydrolysis of an amide bond. / Janda, Kim D.; Schloeder, Diane; Benkovic, Stephen; Lerner, Richard A.

In: Science, Vol. 241, No. 4870, 01.01.1988, p. 1188-1191.

Research output: Contribution to journalArticle

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Janda KD, Schloeder D, Benkovic S, Lerner RA. Induction of an antibody that catalyzes the hydrolysis of an amide bond. Science. 1988 Jan 1;241(4870):1188-1191. https://doi.org/10.1126/science.3413482