Induction of an antibody that catalyzes the hydrolysis of an amide bond

Kim D. Janda, Diane Schloeder, Stephen J. Benkovic, Richard A. Lerner

Research output: Contribution to journalArticle

204 Scopus citations

Abstract

Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

Original languageEnglish (US)
Pages (from-to)1188-1191
Number of pages4
JournalScience
Volume241
Issue number4870
DOIs
StatePublished - Jan 1 1988

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Induction of an antibody that catalyzes the hydrolysis of an amide bond'. Together they form a unique fingerprint.

  • Cite this

    Janda, K. D., Schloeder, D., Benkovic, S. J., & Lerner, R. A. (1988). Induction of an antibody that catalyzes the hydrolysis of an amide bond. Science, 241(4870), 1188-1191. https://doi.org/10.1126/science.3413482