Induction of an antibody that catalyzes the hydrolysis of an amide bond

Kim D. Janda, Diane Schloeder, Stephen J. Benkovic, Richard A. Lerner

Research output: Contribution to journalArticlepeer-review

205 Scopus citations

Abstract

Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

Original languageEnglish (US)
Pages (from-to)1188-1191
Number of pages4
JournalScience
Volume241
Issue number4870
DOIs
StatePublished - Jan 1 1988

All Science Journal Classification (ASJC) codes

  • General

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