Induction of collagenase secretion in human fibroblast cultures by growth-promoting factors

C. C. Chua, D. E. Geiman, G. H. Keller, R. L. Ladda

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

In human foreskin fibroblast cultures two proteins with M(r) 60,000 and 55,000 were found to be induced about 3.5-fold by epidermal growth factor (EGF), platelet-derived growth factor, and β-transforming growth factor. The induced proteins were identified as procollagenases by immunoprecipitation of induced medium with antibodies to purified human fibroblast collagenase. Collagenase enzyme activity in the medium from EGF-treated cultures was also induced at least 3-fold compared to control cultures. Induction of collagenase was dependent upon de novo protein and RNA synthesis and was observed in the medium 10 h after addition of EGF. Although these growth-promoting factors interact with separate membrane receptors, each induced the secretion of a common protein, suggesting that collagenase may be important in some aspect of mitogenesis, cell mobilization, and migration.

Original languageEnglish (US)
Pages (from-to)5213-5216
Number of pages4
JournalJournal of Biological Chemistry
Volume260
Issue number9
StatePublished - 1985

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Collagenases
Fibroblasts
Cell culture
Intercellular Signaling Peptides and Proteins
Epidermal Growth Factor
Proteins
Matrix Metalloproteinase 8
Foreskin
Platelet-Derived Growth Factor
Enzyme activity
Transforming Growth Factors
Immunoprecipitation
Cell Movement
RNA
Membranes
Antibodies
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Chua, C. C., Geiman, D. E., Keller, G. H., & Ladda, R. L. (1985). Induction of collagenase secretion in human fibroblast cultures by growth-promoting factors. Journal of Biological Chemistry, 260(9), 5213-5216.
Chua, C. C. ; Geiman, D. E. ; Keller, G. H. ; Ladda, R. L. / Induction of collagenase secretion in human fibroblast cultures by growth-promoting factors. In: Journal of Biological Chemistry. 1985 ; Vol. 260, No. 9. pp. 5213-5216.
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Chua, CC, Geiman, DE, Keller, GH & Ladda, RL 1985, 'Induction of collagenase secretion in human fibroblast cultures by growth-promoting factors', Journal of Biological Chemistry, vol. 260, no. 9, pp. 5213-5216.

Induction of collagenase secretion in human fibroblast cultures by growth-promoting factors. / Chua, C. C.; Geiman, D. E.; Keller, G. H.; Ladda, R. L.

In: Journal of Biological Chemistry, Vol. 260, No. 9, 1985, p. 5213-5216.

Research output: Contribution to journalArticle

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T1 - Induction of collagenase secretion in human fibroblast cultures by growth-promoting factors

AU - Chua, C. C.

AU - Geiman, D. E.

AU - Keller, G. H.

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