Induction of homologue of Slimb ubiquitin ligase receptor by mitogen signaling

Vladimir Spiegelman, Weigang Tang, Andrew M. Chan, Makoto Igarashi, Stuart A. Aaronson, David A. Sassoon, Masaru Katoh, Thomas J. Slaga, Serge Y. Fuchs

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Homologue of Slimb (HOS) is the substrate-recognizing component of the SCFHOS-Roc1 E3 ubiquitin protein ligase. This ligase mediates ubiquitination of the inhibitor of NF-κB transcription factor (IκB). We have found that HOS is highly expressed in a number of human cancer cell lines. The rates of the HOS gene transcription as well as HOS mRNA and protein levels were up-regulated in cells treated with mitogens or transfected with the inducers of mitogen-activated protein kinase pathway. Conversely, mitogen withdrawal strikingly reduced HOS levels during differentiation of mouse myoblasts. Activators of mitogen-activated protein kinase accelerated IκBα degradation and increased NF-κB transcriptional activity. Inhibition of HOS function via expression of dominant negative HOS (HOSΔF) initiated mouse myoblast differentiation and prevented Ras-mediated acceleration of IκBα degradation as well as NF-κB trans-activation and transformation of NIH3T3 cells. These data link the induction of HOS in proliferating cells with mitogen-signaling-dependent inhibition of cell differentiation and promotion of cell transformation.

Original languageEnglish (US)
Pages (from-to)36624-36630
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number39
DOIs
StatePublished - Sep 27 2002

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Mitogen Receptors
Ligases
Ubiquitin
Mitogens
Mitogen-Activated Protein Kinases
Myoblasts
Cells
Degradation
Ubiquitin-Protein Ligases
Transcription
Ubiquitination
Transcription Factors
Genes
Chemical activation
Cell Differentiation
Messenger RNA
Substrates
Cell Line
Proteins
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Spiegelman, V., Tang, W., Chan, A. M., Igarashi, M., Aaronson, S. A., Sassoon, D. A., ... Fuchs, S. Y. (2002). Induction of homologue of Slimb ubiquitin ligase receptor by mitogen signaling. Journal of Biological Chemistry, 277(39), 36624-36630. https://doi.org/10.1074/jbc.M204524200
Spiegelman, Vladimir ; Tang, Weigang ; Chan, Andrew M. ; Igarashi, Makoto ; Aaronson, Stuart A. ; Sassoon, David A. ; Katoh, Masaru ; Slaga, Thomas J. ; Fuchs, Serge Y. / Induction of homologue of Slimb ubiquitin ligase receptor by mitogen signaling. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 39. pp. 36624-36630.
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abstract = "Homologue of Slimb (HOS) is the substrate-recognizing component of the SCFHOS-Roc1 E3 ubiquitin protein ligase. This ligase mediates ubiquitination of the inhibitor of NF-κB transcription factor (IκB). We have found that HOS is highly expressed in a number of human cancer cell lines. The rates of the HOS gene transcription as well as HOS mRNA and protein levels were up-regulated in cells treated with mitogens or transfected with the inducers of mitogen-activated protein kinase pathway. Conversely, mitogen withdrawal strikingly reduced HOS levels during differentiation of mouse myoblasts. Activators of mitogen-activated protein kinase accelerated IκBα degradation and increased NF-κB transcriptional activity. Inhibition of HOS function via expression of dominant negative HOS (HOSΔF) initiated mouse myoblast differentiation and prevented Ras-mediated acceleration of IκBα degradation as well as NF-κB trans-activation and transformation of NIH3T3 cells. These data link the induction of HOS in proliferating cells with mitogen-signaling-dependent inhibition of cell differentiation and promotion of cell transformation.",
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Spiegelman, V, Tang, W, Chan, AM, Igarashi, M, Aaronson, SA, Sassoon, DA, Katoh, M, Slaga, TJ & Fuchs, SY 2002, 'Induction of homologue of Slimb ubiquitin ligase receptor by mitogen signaling', Journal of Biological Chemistry, vol. 277, no. 39, pp. 36624-36630. https://doi.org/10.1074/jbc.M204524200

Induction of homologue of Slimb ubiquitin ligase receptor by mitogen signaling. / Spiegelman, Vladimir; Tang, Weigang; Chan, Andrew M.; Igarashi, Makoto; Aaronson, Stuart A.; Sassoon, David A.; Katoh, Masaru; Slaga, Thomas J.; Fuchs, Serge Y.

In: Journal of Biological Chemistry, Vol. 277, No. 39, 27.09.2002, p. 36624-36630.

Research output: Contribution to journalArticle

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AU - Tang, Weigang

AU - Chan, Andrew M.

AU - Igarashi, Makoto

AU - Aaronson, Stuart A.

AU - Sassoon, David A.

AU - Katoh, Masaru

AU - Slaga, Thomas J.

AU - Fuchs, Serge Y.

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