Induction of mRNA for chemokines and chemokine receptors by prostaglandin F is dependent upon stage of the porcine corpus luteum and intraluteal progesterone

Wenxiang Luo, Francisco J. Diaz, Milo C. Wiltbank

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

This study tested the hypotheses that prostaglandin (PG) F increases expression of genes related to recruitment of leukocytes in mature but not early corpus luteum (CL) and that insensitivity to PGF action in early CL is dependent on high intraluteal progesterone (P4) concentrations. Experiment 1 examined early (0.5 h) and late (10 h) in vivo effects of PGF on mature (d 17 of pseudopregnancy) and early (d 9) porcine CL. Real-time PCR was used to measure mRNA for chemokines (IL8, CXCL2, CCL2, CCL8, CCL4, CCL11) and chemokine receptors (CCR1, CCR2, CXCR2, CCR5). Western blotting was used to measure protein expression and phosphorylation of nuclear factor-κB proteins. Treatment with PGF for 10 h increased mRNA for almost all of these genes (all expect CXCL2 and CCL11) in d 17 CL but not d 9 CL. Treatment with PGF also led to greater phosphorylation of nuclear factor-κB-1A protein in d 17 than d 9 CL. Experiment 2 had a 2 X 2 factorial design with d 9 gilts treated or not treated with epostane (3β-hydroxysteroid dehydrogenase inhibitor to suppress intraluteal P4) and treated or not treated with PGF. Treatment with PGF (10 h) or epostane alone did not induce expression of any of these genes in d 9 CL. However, PGF + epostane increased expression of all of these genes except CCL11. In conclusion, PGF increases mRNA for chemokines and chemokine receptors in mature CL with similar PGF effects induced in early CL if intraluteal P4 is suppressed prior to PGF treatment.

Original languageEnglish (US)
Pages (from-to)2797-2805
Number of pages9
JournalEndocrinology
Volume152
Issue number7
DOIs
StatePublished - Jul 2011

All Science Journal Classification (ASJC) codes

  • Endocrinology

Fingerprint Dive into the research topics of 'Induction of mRNA for chemokines and chemokine receptors by prostaglandin F<sub>2α</sub> is dependent upon stage of the porcine corpus luteum and intraluteal progesterone'. Together they form a unique fingerprint.

Cite this