Induction of mRNA for chemokines and chemokine receptors by prostaglandin F is dependent upon stage of the porcine corpus luteum and intraluteal progesterone

Wenxiang Luo, Francisco Javier Diaz, Milo C. Wiltbank

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study tested the hypotheses that prostaglandin (PG) F increases expression of genes related to recruitment of leukocytes in mature but not early corpus luteum (CL) and that insensitivity to PGF action in early CL is dependent on high intraluteal progesterone (P4) concentrations. Experiment 1 examined early (0.5 h) and late (10 h) in vivo effects of PGF on mature (d 17 of pseudopregnancy) and early (d 9) porcine CL. Real-time PCR was used to measure mRNA for chemokines (IL8, CXCL2, CCL2, CCL8, CCL4, CCL11) and chemokine receptors (CCR1, CCR2, CXCR2, CCR5). Western blotting was used to measure protein expression and phosphorylation of nuclear factor-κB proteins. Treatment with PGF for 10 h increased mRNA for almost all of these genes (all expect CXCL2 and CCL11) in d 17 CL but not d 9 CL. Treatment with PGF also led to greater phosphorylation of nuclear factor-κB-1A protein in d 17 than d 9 CL. Experiment 2 had a 2 X 2 factorial design with d 9 gilts treated or not treated with epostane (3β-hydroxysteroid dehydrogenase inhibitor to suppress intraluteal P4) and treated or not treated with PGF. Treatment with PGF (10 h) or epostane alone did not induce expression of any of these genes in d 9 CL. However, PGF + epostane increased expression of all of these genes except CCL11. In conclusion, PGF increases mRNA for chemokines and chemokine receptors in mature CL with similar PGF effects induced in early CL if intraluteal P4 is suppressed prior to PGF treatment.

Original languageEnglish (US)
Pages (from-to)2797-2805
Number of pages9
JournalEndocrinology
Volume152
Issue number7
DOIs
StatePublished - Jul 1 2011

Fingerprint

Dinoprost
Chemokine Receptors
Corpus Luteum
Chemokines
Progesterone
Swine
Messenger RNA
Prostaglandins F
Gene Expression
3-Hydroxysteroid Dehydrogenases
Phosphorylation
Pseudopregnancy
Chemokine CXCL2
Proteins
Interleukin-8
Real-Time Polymerase Chain Reaction
Leukocytes
Western Blotting

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

@article{dc988f5d5c0d46de9c56c7d09dce60fe,
title = "Induction of mRNA for chemokines and chemokine receptors by prostaglandin F2α is dependent upon stage of the porcine corpus luteum and intraluteal progesterone",
abstract = "This study tested the hypotheses that prostaglandin (PG) F 2α increases expression of genes related to recruitment of leukocytes in mature but not early corpus luteum (CL) and that insensitivity to PGF2α action in early CL is dependent on high intraluteal progesterone (P4) concentrations. Experiment 1 examined early (0.5 h) and late (10 h) in vivo effects of PGF2α on mature (d 17 of pseudopregnancy) and early (d 9) porcine CL. Real-time PCR was used to measure mRNA for chemokines (IL8, CXCL2, CCL2, CCL8, CCL4, CCL11) and chemokine receptors (CCR1, CCR2, CXCR2, CCR5). Western blotting was used to measure protein expression and phosphorylation of nuclear factor-κB proteins. Treatment with PGF2α for 10 h increased mRNA for almost all of these genes (all expect CXCL2 and CCL11) in d 17 CL but not d 9 CL. Treatment with PGF2α also led to greater phosphorylation of nuclear factor-κB-1A protein in d 17 than d 9 CL. Experiment 2 had a 2 X 2 factorial design with d 9 gilts treated or not treated with epostane (3β-hydroxysteroid dehydrogenase inhibitor to suppress intraluteal P4) and treated or not treated with PGF2α. Treatment with PGF 2α (10 h) or epostane alone did not induce expression of any of these genes in d 9 CL. However, PGF2α + epostane increased expression of all of these genes except CCL11. In conclusion, PGF 2α increases mRNA for chemokines and chemokine receptors in mature CL with similar PGF2α effects induced in early CL if intraluteal P4 is suppressed prior to PGF2α treatment.",
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Induction of mRNA for chemokines and chemokine receptors by prostaglandin F is dependent upon stage of the porcine corpus luteum and intraluteal progesterone. / Luo, Wenxiang; Diaz, Francisco Javier; Wiltbank, Milo C.

In: Endocrinology, Vol. 152, No. 7, 01.07.2011, p. 2797-2805.

Research output: Contribution to journalArticle

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