Induction of single-strand DNA breaks in human cells by H2O2 formed in near-uv (Black Light)-irradiated medium

R. J. Wang, H. N. Ananthaswamy, B. T. Nixon, P. S. Hartman, A. Eisenstark

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30 Scopus citations


When Dulbecco's modified Eagle's medium (depleted of phenol red) was irradiated for up to 3 hr by 4-5 W/m2 black light, hydrogen peroxide (H2O2) was produced. Generation of H2O2 resulted from riboflavin-sensitized photooxidation of tryptophan and tyrosine. Reagent H2O2, or hydrogen peroxide generated in black light-exposed aqueous solutions containing riboflavin and tryptophan, induced 2 x 104 single-strand breaks per 1010 daltons of DNA in intact, physiologically viable human D98/AH2 cells. Concomitant with the single-strand breaks in the cells was loss of cellular reproductive viability. Two cases of photoproducts were identified: H2O2 and non-H2O2. The H2O2 component of the photoproducts was responsible for all the single-strand break induction but for only partial loss of reproductive viability. The non-H2O2 photoproducts, accountable for the remainder of cell lethality, caused no single-strand breaks.

Original languageEnglish (US)
Pages (from-to)269-276
Number of pages8
JournalRadiation research
Issue number2
StatePublished - Jan 1 1980

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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