Induction of single-strand DNA breaks in human cells by H2O2 formed in near-uv (Black Light)-irradiated medium

R. J. Wang, H. N. Ananthaswamy, B. T. Nixon, P. S. Hartman, A. Eisenstark

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

When Dulbecco's modified Eagle's medium (depleted of phenol red) was irradiated for up to 3 hr by 4-5 W/m2 black light, hydrogen peroxide (H2O2) was produced. Generation of H2O2 resulted from riboflavin-sensitized photooxidation of tryptophan and tyrosine. Reagent H2O2, or hydrogen peroxide generated in black light-exposed aqueous solutions containing riboflavin and tryptophan, induced 2 x 104 single-strand breaks per 1010 daltons of DNA in intact, physiologically viable human D98/AH2 cells. Concomitant with the single-strand breaks in the cells was loss of cellular reproductive viability. Two cases of photoproducts were identified: H2O2 and non-H2O2. The H2O2 component of the photoproducts was responsible for all the single-strand break induction but for only partial loss of reproductive viability. The non-H2O2 photoproducts, accountable for the remainder of cell lethality, caused no single-strand breaks.

Original languageEnglish (US)
Pages (from-to)269-276
Number of pages8
JournalRadiation research
Volume82
Issue number2
DOIs
StatePublished - Jan 1 1980

Fingerprint

Single-Stranded DNA Breaks
strands
induction
deoxyribonucleic acid
Riboflavin
riboflavin
Light
Tryptophan
Hydrogen Peroxide
tryptophan
hydrogen peroxide
viability
Phenolsulfonphthalein
Eagles
cells
lethality
Tyrosine
photooxidation
tyrosine
phenols

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Wang, R. J. ; Ananthaswamy, H. N. ; Nixon, B. T. ; Hartman, P. S. ; Eisenstark, A. / Induction of single-strand DNA breaks in human cells by H2O2 formed in near-uv (Black Light)-irradiated medium. In: Radiation research. 1980 ; Vol. 82, No. 2. pp. 269-276.
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Induction of single-strand DNA breaks in human cells by H2O2 formed in near-uv (Black Light)-irradiated medium. / Wang, R. J.; Ananthaswamy, H. N.; Nixon, B. T.; Hartman, P. S.; Eisenstark, A.

In: Radiation research, Vol. 82, No. 2, 01.01.1980, p. 269-276.

Research output: Contribution to journalArticle

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