Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells

Prachya Vichchathorn, Rachaneeporn Jenwithisuk, Surasak Leelaudomlipi, Sumalee Tungpradabkul, Suradej Hongeng, Liwang Cui, Jetsumon Sattabongkot, Rachanee Udomsangpetch

Research output: Contribution to journalArticle

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Abstract

Due to chronic morbidity, the risk of increasing drug resistance and the existence of the hypnozoite stage in Plasmodium vivax malaria, there is a need to find out how hosts develop immunity to compromise the malaria parasites. Here we focused on an in vitro model for immunotherapy and vaccine development. Immunosuppressive mechanisms in malaria include inhibition of T cell response and suppression of dendritic cell function. Using in vitro activation of lymphocytes by malaria antigen-pulsed dendritic cells could overcome the limitation of antigen presentation during acute infections. Here we showed that the sporozoite-pulsed dendritic cell could elicit cytotoxicity against liver stage of P. vivax. Analysis using immunophenotypic markers showed maturation of the dendritic cells and stimulation of cytotoxic T cells. Functional assay of the in vitro-activated cytotoxic T cells showed enhancement of specific killing of the P. vivax exoerythrocytic stages within infected hepatocytes. This model may be useful for vaccine development against human malaria.

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalParasitology International
Volume55
Issue number3
DOIs
StatePublished - Sep 1 2006

Fingerprint

Plasmodium vivax
Dendritic Cells
Malaria
Liver
T-Lymphocytes
Vaccines
Vivax Malaria
Sporozoites
Antigen Presentation
Human Development
Immunosuppressive Agents
Lymphocyte Activation
Drug Resistance
Immunotherapy
Hepatocytes
Immunity
Parasites
Morbidity
Antigens
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Vichchathorn, P., Jenwithisuk, R., Leelaudomlipi, S., Tungpradabkul, S., Hongeng, S., Cui, L., ... Udomsangpetch, R. (2006). Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells. Parasitology International, 55(3), 187-193. https://doi.org/10.1016/j.parint.2006.04.001
Vichchathorn, Prachya ; Jenwithisuk, Rachaneeporn ; Leelaudomlipi, Surasak ; Tungpradabkul, Sumalee ; Hongeng, Suradej ; Cui, Liwang ; Sattabongkot, Jetsumon ; Udomsangpetch, Rachanee. / Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells. In: Parasitology International. 2006 ; Vol. 55, No. 3. pp. 187-193.
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Vichchathorn, P, Jenwithisuk, R, Leelaudomlipi, S, Tungpradabkul, S, Hongeng, S, Cui, L, Sattabongkot, J & Udomsangpetch, R 2006, 'Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells', Parasitology International, vol. 55, no. 3, pp. 187-193. https://doi.org/10.1016/j.parint.2006.04.001

Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells. / Vichchathorn, Prachya; Jenwithisuk, Rachaneeporn; Leelaudomlipi, Surasak; Tungpradabkul, Sumalee; Hongeng, Suradej; Cui, Liwang; Sattabongkot, Jetsumon; Udomsangpetch, Rachanee.

In: Parasitology International, Vol. 55, No. 3, 01.09.2006, p. 187-193.

Research output: Contribution to journalArticle

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