Induction of store-operated calcium entry (SOCE) suppresses glioblastoma growth by inhibiting the Hippo pathway transcriptional coactivators YAP/TAZ

Zhijun Liu, Yiju Wei, Lei Zhang, Patricia P. Yee, Martin Johnson, Xuexin Zhang, Melissa Gulley, Jennifer M. Atkinson, Mohamed Trebak, Hong-Gang Wang, Wei Li

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Glioblastomas (GBM) are the most aggressive brain cancers without effective therapeutics. The Hippo pathway transcriptional coactivators YAP/TAZ were implicated as drivers in GBM progression and could be therapeutic targets. Here we found in an unbiased screen of 1650 compounds that amlodipine is able to inhibit survival of GBM cells by suppressing YAP/TAZ activities. Instead of its known function as an L-type calcium channel blocker, we found that amlodipine is able to activate Ca 2+ entry by enhancing store-operated Ca 2+ entry (SOCE). Amlodipine as well as approaches that cause store depletion and activate SOCE trigger phosphorylation and activation of Lats1/2, which in turn phosphorylate YAP/TAZ and prevent their accumulation in the cell nucleus. Furthermore, we identified that protein kinase C (PKC) beta II is a major mediator of Ca 2+ -induced Lats1/2 activation. Ca 2+ induces accumulation of PKC beta II in an actin cytoskeletal compartment. Such translocation depends on inverted formin-2 (INF2). Depletion of INF2 disrupts both PKC beta II translocation and Lats1/2 activation. Functionally, we found that elevation of cytosolic Ca 2+ or PKC beta II expression inhibits YAP/TAZ-mediated gene transcription. In vivo PKC beta II expression inhibits GBM tumor growth and prolongs mouse survival through inhibition of YAP/TAZ in an orthotopic mouse xenograft model. Our studies indicate that Ca 2+ is a crucial intracellular cue that regulates the Hippo pathway and that triggering SOCE could be a strategy to target YAP/TAZ in GBM.

Original languageEnglish (US)
Pages (from-to)120-139
Number of pages20
JournalOncogene
Volume38
Issue number1
DOIs
StatePublished - Jan 3 2019

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Protein Kinase C beta
Glioblastoma
Amlodipine
Calcium
Growth
L-Type Calcium Channels
Calcium Channel Blockers
Cell Nucleus
Heterografts
Brain Neoplasms
Cues
Actins
Cell Survival
Phosphorylation
Therapeutics
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Liu, Zhijun ; Wei, Yiju ; Zhang, Lei ; Yee, Patricia P. ; Johnson, Martin ; Zhang, Xuexin ; Gulley, Melissa ; Atkinson, Jennifer M. ; Trebak, Mohamed ; Wang, Hong-Gang ; Li, Wei. / Induction of store-operated calcium entry (SOCE) suppresses glioblastoma growth by inhibiting the Hippo pathway transcriptional coactivators YAP/TAZ. In: Oncogene. 2019 ; Vol. 38, No. 1. pp. 120-139.
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abstract = "Glioblastomas (GBM) are the most aggressive brain cancers without effective therapeutics. The Hippo pathway transcriptional coactivators YAP/TAZ were implicated as drivers in GBM progression and could be therapeutic targets. Here we found in an unbiased screen of 1650 compounds that amlodipine is able to inhibit survival of GBM cells by suppressing YAP/TAZ activities. Instead of its known function as an L-type calcium channel blocker, we found that amlodipine is able to activate Ca 2+ entry by enhancing store-operated Ca 2+ entry (SOCE). Amlodipine as well as approaches that cause store depletion and activate SOCE trigger phosphorylation and activation of Lats1/2, which in turn phosphorylate YAP/TAZ and prevent their accumulation in the cell nucleus. Furthermore, we identified that protein kinase C (PKC) beta II is a major mediator of Ca 2+ -induced Lats1/2 activation. Ca 2+ induces accumulation of PKC beta II in an actin cytoskeletal compartment. Such translocation depends on inverted formin-2 (INF2). Depletion of INF2 disrupts both PKC beta II translocation and Lats1/2 activation. Functionally, we found that elevation of cytosolic Ca 2+ or PKC beta II expression inhibits YAP/TAZ-mediated gene transcription. In vivo PKC beta II expression inhibits GBM tumor growth and prolongs mouse survival through inhibition of YAP/TAZ in an orthotopic mouse xenograft model. Our studies indicate that Ca 2+ is a crucial intracellular cue that regulates the Hippo pathway and that triggering SOCE could be a strategy to target YAP/TAZ in GBM.",
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Induction of store-operated calcium entry (SOCE) suppresses glioblastoma growth by inhibiting the Hippo pathway transcriptional coactivators YAP/TAZ. / Liu, Zhijun; Wei, Yiju; Zhang, Lei; Yee, Patricia P.; Johnson, Martin; Zhang, Xuexin; Gulley, Melissa; Atkinson, Jennifer M.; Trebak, Mohamed; Wang, Hong-Gang; Li, Wei.

In: Oncogene, Vol. 38, No. 1, 03.01.2019, p. 120-139.

Research output: Contribution to journalArticle

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