Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells

Shuso Takeda, Mari Harada, Shengzhong Su, Shunsuke Okajima, Hiroko Miyoshi, Kazutaka Yoshida, Hajime Nishimura, Yoshiko Okamoto, Toshiaki Amamoto, Kazuhito Watanabe, Curtis J. Omiecinski, Hironori Aramaki

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


To investigate gene(s) being regulated by Δ9-tetrahydrocannabinol (Δ9-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Δ9-THC for 48 hr at an IC50 concentration of approximately 25 μM. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red O staining after Δ9-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Δ9-THC-regulated gene, and that Δ9-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.

Original languageEnglish (US)
Pages (from-to)305-308
Number of pages4
JournalJournal of Toxicological Sciences
Issue number2
StatePublished - 2013

All Science Journal Classification (ASJC) codes

  • Toxicology

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