Infection of cultured embryo cells of the pacific oyster, Crassostrea gigas, by pantropic retroviral vectors

V. Boulo, J. P. Cadoret, Hiroko Shike, C. Shimizu, A. Miyanohara, J. C. Burns

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The inability to stably introduce and express foreign genes has hampered basic research in molluscan species. We cultured cells from dissociated embryos of the Pacific oyster, Crassostrea gigas, and infected these primary cultures with pantropic retroviral vectors containing the envelope glycoprotein of vesicular stomatitis virus. Luciferase transgene expression mediated by different heterologous promoters was demonstrated for at least 9 d after infection of the cells. Surprisingly, the promoter reproducibly mediating the highest level of luciferase expression was the retroviral promoter (U3 region of long terminal repeat) from the Moloney murine leukemia virus. The infection efficiency using a low multiplicity of infection (0.05) was estimated by quantitative polymerase chain reaction to be between 0.1-0.5%. This system will facilitate studies of gene expression and regulation and should be widely applicable to other molluscan species.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume36
Issue number6
DOIs
StatePublished - Aug 29 2000

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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