Infection of leaves of Arabidopsis thaliana by Botrytis cinerea: Changes in ascorbic acid, free radicals and lipid peroxidation products

I. Muckenschnabel, B. A. Goodman, B. Williamson, G. D. Lyon, Nigel Deighton

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Infection of leaves of Arabidopsis thaliana with conidial suspensions of the necrotrophic pathogen Botrytis cinerea resulted in a large decrease in the level of ascorbic acid and increases in intensity of a single-peak free radical and Fe(III) (g=4.27) signals in electron paramagnetic resonance (EPR) spectra. These changes were not confined to the spreading lesions or associated areas of chlorosis, but extended to other apparently healthy tissues in the infected leaves. They are, therefore, consistent with the existence of high levels of oxidative stress being generated as a result of the infection process. The expected accompanying increases in levels of the aldehydic products of lipid peroxidation, malondialdehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE), were not observed, and in the case of MDA the levels in tissue from infected plants were appreciably lower than in the healthy controls. These last findings are surprising and demonstrate a difference in the response of A. thaliana to infection with B. cinerea compared with tissues from other plant families studied previously.

Original languageEnglish (US)
Pages (from-to)207-214
Number of pages8
JournalJournal of experimental botany
Volume53
Issue number367
DOIs
StatePublished - Jan 1 2002

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Botrytis
Botrytis cinerea
Arabidopsis
Lipid Peroxidation
Ascorbic Acid
Free Radicals
lipid peroxidation
Arabidopsis thaliana
ascorbic acid
Malondialdehyde
malondialdehyde
Infection
infection
Hypochromic Anemia
leaves
lesions (plant)
electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
chlorosis
Suspensions

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

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abstract = "Infection of leaves of Arabidopsis thaliana with conidial suspensions of the necrotrophic pathogen Botrytis cinerea resulted in a large decrease in the level of ascorbic acid and increases in intensity of a single-peak free radical and Fe(III) (g=4.27) signals in electron paramagnetic resonance (EPR) spectra. These changes were not confined to the spreading lesions or associated areas of chlorosis, but extended to other apparently healthy tissues in the infected leaves. They are, therefore, consistent with the existence of high levels of oxidative stress being generated as a result of the infection process. The expected accompanying increases in levels of the aldehydic products of lipid peroxidation, malondialdehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE), were not observed, and in the case of MDA the levels in tissue from infected plants were appreciably lower than in the healthy controls. These last findings are surprising and demonstrate a difference in the response of A. thaliana to infection with B. cinerea compared with tissues from other plant families studied previously.",
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Infection of leaves of Arabidopsis thaliana by Botrytis cinerea : Changes in ascorbic acid, free radicals and lipid peroxidation products. / Muckenschnabel, I.; Goodman, B. A.; Williamson, B.; Lyon, G. D.; Deighton, Nigel.

In: Journal of experimental botany, Vol. 53, No. 367, 01.01.2002, p. 207-214.

Research output: Contribution to journalArticle

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