Infectious dose-dependent accumulation of live highly pathogenic avian influenza H5N1 virus in chicken skeletal muscle—implications for public health

G. Vasudevan, P. R. Vanamayya, S. Nagarajan, K. Rajukumar, S. Suba, G. Venketash, C. Tosh, R. Sood, R. H. Nissly, Suresh Varma Kuchipudi

Research output: Contribution to journalArticle

Abstract

Highly pathogenic avian influenza viruses (HPAIV) of H5N1 subtype are a major global threat to poultry and public health. Export of poultry products, such as chicken and duck meat, is a known source for the cross-boundary spread of HPAI H5N1 viruses. Humans get infected with HPAI H5N1 viruses either by close contact with infected poultry or through consumption of fresh/undercooked poultry meat. Skeletal muscle is the largest soft tissue in chicken that has been shown to contain virus during systemic HPAIV infection and supports productive virus infection. However, the time between infection of a chicken with H5N1 virus and presence of virus in muscle tissue is not yet known. Further, it is also not clear whether chicken infected with low doses of H5N1 virus that cause non-fatal subclinical infections continue to accumulate virus in skeletal muscle. We investigated the amount and duration of virus detection in skeletal muscle of chicken experimentally infected with different doses (102, 103 and 104 EID50) of a HPAI H5N1 virus. Influenza viral antigen could be detected as early as 6 hr after infection and live virus was recovered from 48 hr after infection. Notably, chicken infected with lower levels of HPAI H5N1 virus (i.e., 102 EID50) did not die acutely, but continued to accumulate high levels of H5N1 virus in skeletal muscle until 6 days post-infection. Our data suggest that there is a potential risk of human exposure to H5N1 virus through meat from clinically healthy chicken infected with a low dose of virus. Our results highlight the need to implement rigorous monitoring systems to screen poultry meat from H5N1 endemic countries to limit the global spread of H5N1 viruses.

Original languageEnglish (US)
Pages (from-to)e243-e247
JournalZoonoses and Public Health
Volume65
Issue number1
DOIs
StatePublished - Feb 1 2018

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H5N1 Subtype Influenza A Virus
Influenza in Birds
avian influenza
Orthomyxoviridae
Influenza A virus
Chickens
public health
Public Health
chickens
dosage
Poultry
Meat
viruses
Viruses
Skeletal Muscle
Virus Diseases
skeletal muscle
infection
poultry meat
Infection

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Vasudevan, G. ; Vanamayya, P. R. ; Nagarajan, S. ; Rajukumar, K. ; Suba, S. ; Venketash, G. ; Tosh, C. ; Sood, R. ; Nissly, R. H. ; Kuchipudi, Suresh Varma. / Infectious dose-dependent accumulation of live highly pathogenic avian influenza H5N1 virus in chicken skeletal muscle—implications for public health. In: Zoonoses and Public Health. 2018 ; Vol. 65, No. 1. pp. e243-e247.
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abstract = "Highly pathogenic avian influenza viruses (HPAIV) of H5N1 subtype are a major global threat to poultry and public health. Export of poultry products, such as chicken and duck meat, is a known source for the cross-boundary spread of HPAI H5N1 viruses. Humans get infected with HPAI H5N1 viruses either by close contact with infected poultry or through consumption of fresh/undercooked poultry meat. Skeletal muscle is the largest soft tissue in chicken that has been shown to contain virus during systemic HPAIV infection and supports productive virus infection. However, the time between infection of a chicken with H5N1 virus and presence of virus in muscle tissue is not yet known. Further, it is also not clear whether chicken infected with low doses of H5N1 virus that cause non-fatal subclinical infections continue to accumulate virus in skeletal muscle. We investigated the amount and duration of virus detection in skeletal muscle of chicken experimentally infected with different doses (102, 103 and 104 EID50) of a HPAI H5N1 virus. Influenza viral antigen could be detected as early as 6 hr after infection and live virus was recovered from 48 hr after infection. Notably, chicken infected with lower levels of HPAI H5N1 virus (i.e., 102 EID50) did not die acutely, but continued to accumulate high levels of H5N1 virus in skeletal muscle until 6 days post-infection. Our data suggest that there is a potential risk of human exposure to H5N1 virus through meat from clinically healthy chicken infected with a low dose of virus. Our results highlight the need to implement rigorous monitoring systems to screen poultry meat from H5N1 endemic countries to limit the global spread of H5N1 viruses.",
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Vasudevan, G, Vanamayya, PR, Nagarajan, S, Rajukumar, K, Suba, S, Venketash, G, Tosh, C, Sood, R, Nissly, RH & Kuchipudi, SV 2018, 'Infectious dose-dependent accumulation of live highly pathogenic avian influenza H5N1 virus in chicken skeletal muscle—implications for public health', Zoonoses and Public Health, vol. 65, no. 1, pp. e243-e247. https://doi.org/10.1111/zph.12406

Infectious dose-dependent accumulation of live highly pathogenic avian influenza H5N1 virus in chicken skeletal muscle—implications for public health. / Vasudevan, G.; Vanamayya, P. R.; Nagarajan, S.; Rajukumar, K.; Suba, S.; Venketash, G.; Tosh, C.; Sood, R.; Nissly, R. H.; Kuchipudi, Suresh Varma.

In: Zoonoses and Public Health, Vol. 65, No. 1, 01.02.2018, p. e243-e247.

Research output: Contribution to journalArticle

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T1 - Infectious dose-dependent accumulation of live highly pathogenic avian influenza H5N1 virus in chicken skeletal muscle—implications for public health

AU - Vasudevan, G.

AU - Vanamayya, P. R.

AU - Nagarajan, S.

AU - Rajukumar, K.

AU - Suba, S.

AU - Venketash, G.

AU - Tosh, C.

AU - Sood, R.

AU - Nissly, R. H.

AU - Kuchipudi, Suresh Varma

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N2 - Highly pathogenic avian influenza viruses (HPAIV) of H5N1 subtype are a major global threat to poultry and public health. Export of poultry products, such as chicken and duck meat, is a known source for the cross-boundary spread of HPAI H5N1 viruses. Humans get infected with HPAI H5N1 viruses either by close contact with infected poultry or through consumption of fresh/undercooked poultry meat. Skeletal muscle is the largest soft tissue in chicken that has been shown to contain virus during systemic HPAIV infection and supports productive virus infection. However, the time between infection of a chicken with H5N1 virus and presence of virus in muscle tissue is not yet known. Further, it is also not clear whether chicken infected with low doses of H5N1 virus that cause non-fatal subclinical infections continue to accumulate virus in skeletal muscle. We investigated the amount and duration of virus detection in skeletal muscle of chicken experimentally infected with different doses (102, 103 and 104 EID50) of a HPAI H5N1 virus. Influenza viral antigen could be detected as early as 6 hr after infection and live virus was recovered from 48 hr after infection. Notably, chicken infected with lower levels of HPAI H5N1 virus (i.e., 102 EID50) did not die acutely, but continued to accumulate high levels of H5N1 virus in skeletal muscle until 6 days post-infection. Our data suggest that there is a potential risk of human exposure to H5N1 virus through meat from clinically healthy chicken infected with a low dose of virus. Our results highlight the need to implement rigorous monitoring systems to screen poultry meat from H5N1 endemic countries to limit the global spread of H5N1 viruses.

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