Influence of cation concentration on the sialidase activity of neuronal synaptic membranes

Cara-Lynne Schengrund, J. T. Nelson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Low Na+ (5×10-5M) and higher K+ or divalent cation concentration (5×10-4M) fully activate synaptic membrane sialidase towards exogenous gangliosides. As the concentration of Ca++ or Zn++ is increased above 510-4M, exogenous sialidase activity is inhibited. Increased Na+ or K+ concentrations do not give this effect. Activity towards endogenous gangliosides is not controlled by external cations. Both endogenous and exogenous activities are optimum over a narrow pH range, 3.9 to 4.5, and activity is low above 6 or below 2. These results suggest that pH and ionic flux may regulate the density of fixed negative charge due to bound sialic acid in the synapse, and influence synaptic transmission, by controlling the separate actions of synaptic membrane sialidase.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume63
Issue number1
DOIs
StatePublished - Mar 3 1975

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Synaptic Membranes
Neuraminidase
Cations
Gangliosides
Membranes
Divalent Cations
N-Acetylneuraminic Acid
Synaptic Transmission
Synapses
Fluxes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Influence of cation concentration on the sialidase activity of neuronal synaptic membranes. / Schengrund, Cara-Lynne; Nelson, J. T.

In: Biochemical and Biophysical Research Communications, Vol. 63, No. 1, 03.03.1975, p. 217-223.

Research output: Contribution to journalArticle

TY - JOUR

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