Influence of cation concentration on the sialidase activity of neuronal synaptic membranes

C. L. Schengrund; J. T. Nelson

doi:10.1016/S0006-291X(75)80032-5

Influence of cation concentration on the sialidase activity of neuronal synaptic membranes

C. L. Schengrund, J. T. Nelson

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

Low Na⁺ (5×10^-5M) and higher K⁺ or divalent cation concentration (5×10^-4M) fully activate synaptic membrane sialidase towards exogenous gangliosides. As the concentration of Ca⁺⁺ or Zn⁺⁺ is increased above 510^-4M, exogenous sialidase activity is inhibited. Increased Na⁺ or K⁺ concentrations do not give this effect. Activity towards endogenous gangliosides is not controlled by external cations. Both endogenous and exogenous activities are optimum over a narrow pH range, 3.9 to 4.5, and activity is low above 6 or below 2. These results suggest that pH and ionic flux may regulate the density of fixed negative charge due to bound sialic acid in the synapse, and influence synaptic transmission, by controlling the separate actions of synaptic membrane sialidase.

Original language	English (US)
Pages (from-to)	217-223
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(75)80032-5
State	Published - Mar 3 1975

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(75)80032-5

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title = "Influence of cation concentration on the sialidase activity of neuronal synaptic membranes",

abstract = "Low Na+ (5×10-5M) and higher K+ or divalent cation concentration (5×10-4M) fully activate synaptic membrane sialidase towards exogenous gangliosides. As the concentration of Ca++ or Zn++ is increased above 510-4M, exogenous sialidase activity is inhibited. Increased Na+ or K+ concentrations do not give this effect. Activity towards endogenous gangliosides is not controlled by external cations. Both endogenous and exogenous activities are optimum over a narrow pH range, 3.9 to 4.5, and activity is low above 6 or below 2. These results suggest that pH and ionic flux may regulate the density of fixed negative charge due to bound sialic acid in the synapse, and influence synaptic transmission, by controlling the separate actions of synaptic membrane sialidase.",

author = "Schengrund, {C. L.} and Nelson, {J. T.}",

note = "Funding Information: This work was supported by Public Health Service Research Grant CA14319 from the National Cancer Institute and by Grant NS08258 from the National Institute of Neurological Diseases and Stroke.",

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AU - Schengrund, C. L.

AU - Nelson, J. T.

N1 - Funding Information: This work was supported by Public Health Service Research Grant CA14319 from the National Cancer Institute and by Grant NS08258 from the National Institute of Neurological Diseases and Stroke.

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N2 - Low Na+ (5×10-5M) and higher K+ or divalent cation concentration (5×10-4M) fully activate synaptic membrane sialidase towards exogenous gangliosides. As the concentration of Ca++ or Zn++ is increased above 510-4M, exogenous sialidase activity is inhibited. Increased Na+ or K+ concentrations do not give this effect. Activity towards endogenous gangliosides is not controlled by external cations. Both endogenous and exogenous activities are optimum over a narrow pH range, 3.9 to 4.5, and activity is low above 6 or below 2. These results suggest that pH and ionic flux may regulate the density of fixed negative charge due to bound sialic acid in the synapse, and influence synaptic transmission, by controlling the separate actions of synaptic membrane sialidase.

AB - Low Na+ (5×10-5M) and higher K+ or divalent cation concentration (5×10-4M) fully activate synaptic membrane sialidase towards exogenous gangliosides. As the concentration of Ca++ or Zn++ is increased above 510-4M, exogenous sialidase activity is inhibited. Increased Na+ or K+ concentrations do not give this effect. Activity towards endogenous gangliosides is not controlled by external cations. Both endogenous and exogenous activities are optimum over a narrow pH range, 3.9 to 4.5, and activity is low above 6 or below 2. These results suggest that pH and ionic flux may regulate the density of fixed negative charge due to bound sialic acid in the synapse, and influence synaptic transmission, by controlling the separate actions of synaptic membrane sialidase.

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Influence of cation concentration on the sialidase activity of neuronal synaptic membranes

Abstract

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