TY - JOUR
T1 - Influence of Escherichia coli hydrogenases on hydrogen fermentation from glycerol
AU - Sanchez-Torres, Viviana
AU - Mohd Yusoff, Mohd Zulkhairi
AU - Nakano, Chieri
AU - Maeda, Toshinari
AU - Ogawa, Hiroaki I.
AU - Wood, Thomas K.
N1 - Funding Information:
This research was supported by the National Science Foundation ( CBET-0753702 ), the Grant-In-Aid for Young Scientists (B) of Japan Society for the Promotion of Science ( 22780070 ), and the Sasakawa Scientific Research Grant (24-521). We are grateful for the Keio strains provided by the National Institute of Genetics, Japan.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/4/1
Y1 - 2013/4/1
N2 - Since the actual role of Escherichia coli hydrogenases on fermentation from glycerol has not been clear, we evaluated the effect of inactivation of each E. coli hydrogenase on cell growth, hydrogen production, organic acids production, and ethanol production. Inactivation of hydrogenase 2 and hydrogenase 3 reduced cell growth, hydrogen and succinate production as well as glycerol utilization while acetate increased. Inactivation of hydrogenase 2 in minimal medium at pH 7.5 impaired hydrogen production, but no significant effect occurred at pH 6.5 or in complex medium. Inactivation of hydrogenase 3 impaired hydrogen production in minimal and rich medium, pH 6.5 and pH 7.5 accumulating formate in all conditions. Therefore during fermentation from glycerol, hydrogenase 3 is the main hydrogenase with hydrogen synthesis activity through the formate hydrogen lyase complex. Hydrogenase 2 seems mainly required for optimum glycerol metabolism rather than hydrogen synthesis. There were no significant impacts by inactivating hydrogenase 1 and hydrogenase 4.
AB - Since the actual role of Escherichia coli hydrogenases on fermentation from glycerol has not been clear, we evaluated the effect of inactivation of each E. coli hydrogenase on cell growth, hydrogen production, organic acids production, and ethanol production. Inactivation of hydrogenase 2 and hydrogenase 3 reduced cell growth, hydrogen and succinate production as well as glycerol utilization while acetate increased. Inactivation of hydrogenase 2 in minimal medium at pH 7.5 impaired hydrogen production, but no significant effect occurred at pH 6.5 or in complex medium. Inactivation of hydrogenase 3 impaired hydrogen production in minimal and rich medium, pH 6.5 and pH 7.5 accumulating formate in all conditions. Therefore during fermentation from glycerol, hydrogenase 3 is the main hydrogenase with hydrogen synthesis activity through the formate hydrogen lyase complex. Hydrogenase 2 seems mainly required for optimum glycerol metabolism rather than hydrogen synthesis. There were no significant impacts by inactivating hydrogenase 1 and hydrogenase 4.
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U2 - 10.1016/j.ijhydene.2013.01.031
DO - 10.1016/j.ijhydene.2013.01.031
M3 - Article
AN - SCOPUS:84875209994
VL - 38
SP - 3905
EP - 3912
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
SN - 0360-3199
IS - 10
ER -