Influence of indigenous eukaryotic microbial communities on the reduction of Escherichia coli O157:H7 in compost slurry

Amrita Puri, Edward G. Dudley

Research output: Contribution to journalLetter

7 Citations (Scopus)

Abstract

Compost made from livestock manure is commonly used as a crop fertilizer and serves as a possible vehicle for the transmission of Escherichia coli O157:H7 to fresh produce. In this study, we hypothesized that the indigenous microbial communities present in composts adversely affects the survival of E. coli O157:H7. Escherichia coli O157:H7 was spiked into compost slurry and incubated at 25 °C. Escherichia coli O157:H7 exhibited a c. 4 log10 reduction over 16 days. When compost was supplemented with the eukaryotic inhibitor cycloheximide, there was a minimal decrease in E. coli O157:H7 counts over the same time period. Analysis of microbial communities present in the compost with denaturing gradient gel electrophoresis (DGGE) suggested minor differences in the fungal communities present in cycloheximide-treated compost, compared with untreated compost over a period of 12 days at 25 °C. However, the DGGE profiles of protists showed drastic differences in community complexity. Clone library sequence analysis of protist populations revealed significantly different species composition between treatment and control samples at different time points. This suggests that predation of E. coli O157:H7 by protists might be a potential mechanism for reducing E. coli O157:H7 in compost materials.

Original languageEnglish (US)
Pages (from-to)148-154
Number of pages7
JournalFEMS Microbiology Letters
Volume313
Issue number2
DOIs
StatePublished - Dec 1 2010

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Escherichia coli O157
Soil
Denaturing Gradient Gel Electrophoresis
Cycloheximide
Manure
Fertilizers
Livestock
Sequence Analysis
Clone Cells

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

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Influence of indigenous eukaryotic microbial communities on the reduction of Escherichia coli O157:H7 in compost slurry. / Puri, Amrita; Dudley, Edward G.

In: FEMS Microbiology Letters, Vol. 313, No. 2, 01.12.2010, p. 148-154.

Research output: Contribution to journalLetter

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