Influence of monocropping brown stem rot-resistant and -susceptible soybean accessions on soil and stem populations of phialophora gregata f. sp. sojae

T. J. Hughes, N. C. Koval, Paul Esker, C. R. Grau

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Abstract

Hughes, T. J., Koval, N. C., Esker, P. D., and Grau, C. R. 2009. Influence of monocropping brown stem rot-resistant and -susceptible soybean accessions on soil and stem populations of Phialophora gregata f. sp. sojae. Plant Dis. 93:1050-1058. Brown stem rot (BSR)-resistant and -susceptible soybean accessions were continuously cropped in an area never previously seeded to soybean to study the influence of monocultures on soil and stem populations of Phialophora gregata f. sp. sojae. P. gregata f. sp. sojae population size and genotype composition were determined by dilution plating, isolation of P. gregata f. sp. sojae and standard polymerase chain reaction (PCR), and by quantitative real-time PCR (q-PCR. In general, the sizes of P. gregata f. sp. sojae populations in soil were similar regardless of monoculture. The percentage of P. gregata f. sp. sojae genotype B was greater than A in soil following the monoculture of both BSR-susceptible and -resistant soybean accessions. Following the monoculture of a BSR-resistant accession, the percentage of P. gregata f. sp. sojae genotype B was greater than A. Overall, P. gregata f. sp. sojae populations in stems of a BSR-susceptible accession were greater than those in stems of a BSR-resistant accession. P. gregata f. sp. sojae genotype B was detected more often than A in stems of both resistant and susceptible accessions planted following a BSR-resistant monoculture. P. gregata f. sp. sojae genotype B was also detected more often than A in stems of a BSR-resistant accession planted following a BSRsusceptible monoculture. P. gregata f. sp. sojae genotypes A and B were isolated at similar frequencies from stems of a BSR-susceptible accession planted following a BSR-susceptible monoculture. However, q-PCR results indicate that the percentage of P. gregata f. sp. sojae genotype A was greater than B in stems of a BSR-susceptible accession planted following a BSR-susceptible monoculture. Among BSR-susceptible accessions, those with the soybean cyst nematode (SCN)-resistant cv. Peking in their parentage had the largest populations of P. gregata f. sp. sojae and a greater percentage of P. gregata f. sp. sojae genotype B. Similar results were observed for BSR-resistant accessions derived from SCN-resistant PI 88788.

Original languageEnglish (US)
Pages (from-to)1050-1058
Number of pages9
JournalPlant disease
Volume93
Issue number10
DOIs
StatePublished - Oct 1 2009

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Cadophora gregata
stem rot
continuous cropping
soybeans
stems
soil
genotype
Heterodera glycines
polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{3249b7f642a348d885ac484e9a3650e4,
title = "Influence of monocropping brown stem rot-resistant and -susceptible soybean accessions on soil and stem populations of phialophora gregata f. sp. sojae",
abstract = "Hughes, T. J., Koval, N. C., Esker, P. D., and Grau, C. R. 2009. Influence of monocropping brown stem rot-resistant and -susceptible soybean accessions on soil and stem populations of Phialophora gregata f. sp. sojae. Plant Dis. 93:1050-1058. Brown stem rot (BSR)-resistant and -susceptible soybean accessions were continuously cropped in an area never previously seeded to soybean to study the influence of monocultures on soil and stem populations of Phialophora gregata f. sp. sojae. P. gregata f. sp. sojae population size and genotype composition were determined by dilution plating, isolation of P. gregata f. sp. sojae and standard polymerase chain reaction (PCR), and by quantitative real-time PCR (q-PCR. In general, the sizes of P. gregata f. sp. sojae populations in soil were similar regardless of monoculture. The percentage of P. gregata f. sp. sojae genotype B was greater than A in soil following the monoculture of both BSR-susceptible and -resistant soybean accessions. Following the monoculture of a BSR-resistant accession, the percentage of P. gregata f. sp. sojae genotype B was greater than A. Overall, P. gregata f. sp. sojae populations in stems of a BSR-susceptible accession were greater than those in stems of a BSR-resistant accession. P. gregata f. sp. sojae genotype B was detected more often than A in stems of both resistant and susceptible accessions planted following a BSR-resistant monoculture. P. gregata f. sp. sojae genotype B was also detected more often than A in stems of a BSR-resistant accession planted following a BSRsusceptible monoculture. P. gregata f. sp. sojae genotypes A and B were isolated at similar frequencies from stems of a BSR-susceptible accession planted following a BSR-susceptible monoculture. However, q-PCR results indicate that the percentage of P. gregata f. sp. sojae genotype A was greater than B in stems of a BSR-susceptible accession planted following a BSR-susceptible monoculture. Among BSR-susceptible accessions, those with the soybean cyst nematode (SCN)-resistant cv. Peking in their parentage had the largest populations of P. gregata f. sp. sojae and a greater percentage of P. gregata f. sp. sojae genotype B. Similar results were observed for BSR-resistant accessions derived from SCN-resistant PI 88788.",
author = "Hughes, {T. J.} and Koval, {N. C.} and Paul Esker and Grau, {C. R.}",
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Influence of monocropping brown stem rot-resistant and -susceptible soybean accessions on soil and stem populations of phialophora gregata f. sp. sojae. / Hughes, T. J.; Koval, N. C.; Esker, Paul; Grau, C. R.

In: Plant disease, Vol. 93, No. 10, 01.10.2009, p. 1050-1058.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Influence of monocropping brown stem rot-resistant and -susceptible soybean accessions on soil and stem populations of phialophora gregata f. sp. sojae

AU - Hughes, T. J.

AU - Koval, N. C.

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