Influence of short-lasting bilateral clamping of carotid arteries (BCCA) on GABA turnover in rat brain structures

M. Sieklucka, W. Löscher, C. Heim, K. H. Sontag

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have previously shown that short-lasting reduction of cerebral blood flow by bilateral clamping of carotid arteries (BCCA) results in long-lasting increase in regional GABA concentration and decrease in seizure susceptibility in rats. In the present experiments, the effect of BCCA on GABA turnover and the enzymes involved in GABA synthesis and degradation were studied in rats. Regional GABA turnover was measured by means of GABA accumulation induced by the GABA-transaminase (GABA-T) inhibitor aminooxyacetic acid (AOAA). Fourteen days after BCCA, GABA turnover was significantly increased in hippocampus, substantia nigra and cortex, but not different from sham-operated controls in several other brain regions, including striatum, hypothalamus and cerebellum. The activity of glutamate decarboxylase (GAD) measured ex vivo did not show any changes in investigated structures, while the activity of GABA-T was slightly increased in hippocampus. The increased GABA turnover in some brain regions may explain our previous findings of increased GABA content in these brain regions and decreased sensitivity of BCCA treated animals to the GABAA-receptor antagonist bicuculline.

Original languageEnglish (US)
Pages (from-to)367-372
Number of pages6
JournalNeurochemical Research
Volume19
Issue number3
DOIs
StatePublished - Mar 1 1994

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Carotid Arteries
Constriction
gamma-Aminobutyric Acid
Rats
Brain
4-Aminobutyrate Transaminase
Hippocampus
Cerebrovascular Circulation
Aminooxyacetic Acid
GABA-A Receptor Antagonists
Glutamate Decarboxylase
Bicuculline
Substantia Nigra
Cerebellum
Hypothalamus
Animals
Seizures
Blood
Degradation
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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title = "Influence of short-lasting bilateral clamping of carotid arteries (BCCA) on GABA turnover in rat brain structures",
abstract = "We have previously shown that short-lasting reduction of cerebral blood flow by bilateral clamping of carotid arteries (BCCA) results in long-lasting increase in regional GABA concentration and decrease in seizure susceptibility in rats. In the present experiments, the effect of BCCA on GABA turnover and the enzymes involved in GABA synthesis and degradation were studied in rats. Regional GABA turnover was measured by means of GABA accumulation induced by the GABA-transaminase (GABA-T) inhibitor aminooxyacetic acid (AOAA). Fourteen days after BCCA, GABA turnover was significantly increased in hippocampus, substantia nigra and cortex, but not different from sham-operated controls in several other brain regions, including striatum, hypothalamus and cerebellum. The activity of glutamate decarboxylase (GAD) measured ex vivo did not show any changes in investigated structures, while the activity of GABA-T was slightly increased in hippocampus. The increased GABA turnover in some brain regions may explain our previous findings of increased GABA content in these brain regions and decreased sensitivity of BCCA treated animals to the GABAA-receptor antagonist bicuculline.",
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Influence of short-lasting bilateral clamping of carotid arteries (BCCA) on GABA turnover in rat brain structures. / Sieklucka, M.; Löscher, W.; Heim, C.; Sontag, K. H.

In: Neurochemical Research, Vol. 19, No. 3, 01.03.1994, p. 367-372.

Research output: Contribution to journalArticle

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