Influx and efflux of P in roots of intact maize plants: Double-labeling with 32P and 33P

George C. Elliott, Jonathan Lynch, Andre Läuchli

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Rates of P influx and efflux were determined in whole plants at ambient P concentrations comparable to those found in soil solutions. Maize (Zea mays L. var NC+59) seedlings were trimmed (endosperm and adventitious roots removed) and grown in a greenhouse in solution cultures at P concentrations of approximately 0.4 and 1.8 micromolar. Roots of intact plants previously exposed to 32P-labeled solutions at 0.2 and 2.0 micromolar P for 48 hours were rinsed 10 minutes in P-free solution and exposed to 33P solutions at 0.2 and 2.0 micromolar for 10 minutes. Net depletion of 33P from and appearance of 32P in the ambient solution were used to measure influx and efflux. The ration of 32P efflux to 33P influx was about 0.68 at 0.2 micromolar and 0.08 at 2.0 micromolar. When plants were allowed to deplete P from solutions, the P concentration in the medium dropped to about 0.15 micromolar within 24 hours and 0.05 micromolar within 60 hours. Results indicate that P efflux is a substantial component of net P accumulation at P concentrations normally found in soil solutions.

Original languageEnglish (US)
Pages (from-to)336-341
Number of pages6
JournalPlant physiology
Volume76
Issue number2
DOIs
StatePublished - Jan 1 1984

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Zea mays
corn
soil solution
Soil
adventitious roots
Endosperm
Plant Roots
endosperm
Seedlings
greenhouses
seedlings

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Elliott, George C. ; Lynch, Jonathan ; Läuchli, Andre. / Influx and efflux of P in roots of intact maize plants : Double-labeling with 32P and 33P. In: Plant physiology. 1984 ; Vol. 76, No. 2. pp. 336-341.
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Influx and efflux of P in roots of intact maize plants : Double-labeling with 32P and 33P. / Elliott, George C.; Lynch, Jonathan; Läuchli, Andre.

In: Plant physiology, Vol. 76, No. 2, 01.01.1984, p. 336-341.

Research output: Contribution to journalArticle

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AB - Rates of P influx and efflux were determined in whole plants at ambient P concentrations comparable to those found in soil solutions. Maize (Zea mays L. var NC+59) seedlings were trimmed (endosperm and adventitious roots removed) and grown in a greenhouse in solution cultures at P concentrations of approximately 0.4 and 1.8 micromolar. Roots of intact plants previously exposed to 32P-labeled solutions at 0.2 and 2.0 micromolar P for 48 hours were rinsed 10 minutes in P-free solution and exposed to 33P solutions at 0.2 and 2.0 micromolar for 10 minutes. Net depletion of 33P from and appearance of 32P in the ambient solution were used to measure influx and efflux. The ration of 32P efflux to 33P influx was about 0.68 at 0.2 micromolar and 0.08 at 2.0 micromolar. When plants were allowed to deplete P from solutions, the P concentration in the medium dropped to about 0.15 micromolar within 24 hours and 0.05 micromolar within 60 hours. Results indicate that P efflux is a substantial component of net P accumulation at P concentrations normally found in soil solutions.

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