Inhibition by diltiazem of norepinephrine release from sympathetic nerves in the rabbit pulmonary artery

Robert Zelis, Thomas Wichmann, Klaus Starke

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to determine if the calcium blocker diltiazem could alter excitation-secretion coupling in vascular sympathetic nerves, superfused rabbit pulmonary arterial strips were preincubated with 3H-norepinephrine and stimulated electrically (2-8 Hz) and by KG (60 mM). Diltiazem significantly inhibited tension development with 4 Hz (1.5 × 10-5M) and 8 Hz (1.5 × 10-6, 1.5 × 10-5 M) stimuli, but only at 1.5 × 10-5 M did diltiazem inhibit the 3H overflow evoked by an 8-Hz stimulus. No inhibition of 3H overflow was noted with the 2 and 4 Hz stimuli. With an 8-Hz stimulus the inhibition of tension development (45.4%) by diltiazem (1.5 × 10-5 M) was significantly greater than the inhibition of 3H overflow (33.8%). A similar relationship was noted with 60 mM KCl stimulation. These data suggest that excitation-secretion coupling in vascular sympathetic nerves can be inhibited by diltiazem.

Original languageEnglish (US)
Pages (from-to)268-277
Number of pages10
JournalPharmacology
Volume31
Issue number5
DOIs
StatePublished - Jan 1 1985

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Diltiazem
Pulmonary Artery
Norepinephrine
Rabbits
Blood Vessels
Calcium
Lung

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

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abstract = "In order to determine if the calcium blocker diltiazem could alter excitation-secretion coupling in vascular sympathetic nerves, superfused rabbit pulmonary arterial strips were preincubated with 3H-norepinephrine and stimulated electrically (2-8 Hz) and by KG (60 mM). Diltiazem significantly inhibited tension development with 4 Hz (1.5 × 10-5M) and 8 Hz (1.5 × 10-6, 1.5 × 10-5 M) stimuli, but only at 1.5 × 10-5 M did diltiazem inhibit the 3H overflow evoked by an 8-Hz stimulus. No inhibition of 3H overflow was noted with the 2 and 4 Hz stimuli. With an 8-Hz stimulus the inhibition of tension development (45.4{\%}) by diltiazem (1.5 × 10-5 M) was significantly greater than the inhibition of 3H overflow (33.8{\%}). A similar relationship was noted with 60 mM KCl stimulation. These data suggest that excitation-secretion coupling in vascular sympathetic nerves can be inhibited by diltiazem.",
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Inhibition by diltiazem of norepinephrine release from sympathetic nerves in the rabbit pulmonary artery. / Zelis, Robert; Wichmann, Thomas; Starke, Klaus.

In: Pharmacology, Vol. 31, No. 5, 01.01.1985, p. 268-277.

Research output: Contribution to journalArticle

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AU - Starke, Klaus

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N2 - In order to determine if the calcium blocker diltiazem could alter excitation-secretion coupling in vascular sympathetic nerves, superfused rabbit pulmonary arterial strips were preincubated with 3H-norepinephrine and stimulated electrically (2-8 Hz) and by KG (60 mM). Diltiazem significantly inhibited tension development with 4 Hz (1.5 × 10-5M) and 8 Hz (1.5 × 10-6, 1.5 × 10-5 M) stimuli, but only at 1.5 × 10-5 M did diltiazem inhibit the 3H overflow evoked by an 8-Hz stimulus. No inhibition of 3H overflow was noted with the 2 and 4 Hz stimuli. With an 8-Hz stimulus the inhibition of tension development (45.4%) by diltiazem (1.5 × 10-5 M) was significantly greater than the inhibition of 3H overflow (33.8%). A similar relationship was noted with 60 mM KCl stimulation. These data suggest that excitation-secretion coupling in vascular sympathetic nerves can be inhibited by diltiazem.

AB - In order to determine if the calcium blocker diltiazem could alter excitation-secretion coupling in vascular sympathetic nerves, superfused rabbit pulmonary arterial strips were preincubated with 3H-norepinephrine and stimulated electrically (2-8 Hz) and by KG (60 mM). Diltiazem significantly inhibited tension development with 4 Hz (1.5 × 10-5M) and 8 Hz (1.5 × 10-6, 1.5 × 10-5 M) stimuli, but only at 1.5 × 10-5 M did diltiazem inhibit the 3H overflow evoked by an 8-Hz stimulus. No inhibition of 3H overflow was noted with the 2 and 4 Hz stimuli. With an 8-Hz stimulus the inhibition of tension development (45.4%) by diltiazem (1.5 × 10-5 M) was significantly greater than the inhibition of 3H overflow (33.8%). A similar relationship was noted with 60 mM KCl stimulation. These data suggest that excitation-secretion coupling in vascular sympathetic nerves can be inhibited by diltiazem.

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