Inhibition of aerobic respiration and dissimilatory perchlorate reduction using cyanide

Yanguang Song, Bruce Ernest Logan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 μM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity of chlorite dismutase. When oxygen accumulation was prevented by adding an oxygen scavenger (Oxyrase or l-cysteine), however, cells completely reduced perchlorate in the presence of cyanide. It was concluded that the inhibition of dissimilative perchlorate reduction by cyanide at this concentration was a consequence of oxygen accumulation, not inhibition of the enzymes used for perchlorate reduction. This finding on the effect of cyanide on respiratory enzymes provides a new method to control and study respiratory enzymes used for perchlorate reduction.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalFEMS Microbiology Letters
Volume239
Issue number2
DOIs
StatePublished - Oct 15 2004

Fingerprint

Cyanides
Respiration
Chlorates
Oxygen
chlorite dismutase
Enzymes
Cysteine
perchlorate
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

@article{5bc160951c8b4201bfccbff69d5a8ebc,
title = "Inhibition of aerobic respiration and dissimilatory perchlorate reduction using cyanide",
abstract = "The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 μM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity of chlorite dismutase. When oxygen accumulation was prevented by adding an oxygen scavenger (Oxyrase or l-cysteine), however, cells completely reduced perchlorate in the presence of cyanide. It was concluded that the inhibition of dissimilative perchlorate reduction by cyanide at this concentration was a consequence of oxygen accumulation, not inhibition of the enzymes used for perchlorate reduction. This finding on the effect of cyanide on respiratory enzymes provides a new method to control and study respiratory enzymes used for perchlorate reduction.",
author = "Yanguang Song and Logan, {Bruce Ernest}",
year = "2004",
month = "10",
day = "15",
doi = "10.1016/j.femsle.2004.08.031",
language = "English (US)",
volume = "239",
pages = "229--234",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

Inhibition of aerobic respiration and dissimilatory perchlorate reduction using cyanide. / Song, Yanguang; Logan, Bruce Ernest.

In: FEMS Microbiology Letters, Vol. 239, No. 2, 15.10.2004, p. 229-234.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibition of aerobic respiration and dissimilatory perchlorate reduction using cyanide

AU - Song, Yanguang

AU - Logan, Bruce Ernest

PY - 2004/10/15

Y1 - 2004/10/15

N2 - The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 μM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity of chlorite dismutase. When oxygen accumulation was prevented by adding an oxygen scavenger (Oxyrase or l-cysteine), however, cells completely reduced perchlorate in the presence of cyanide. It was concluded that the inhibition of dissimilative perchlorate reduction by cyanide at this concentration was a consequence of oxygen accumulation, not inhibition of the enzymes used for perchlorate reduction. This finding on the effect of cyanide on respiratory enzymes provides a new method to control and study respiratory enzymes used for perchlorate reduction.

AB - The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 μM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity of chlorite dismutase. When oxygen accumulation was prevented by adding an oxygen scavenger (Oxyrase or l-cysteine), however, cells completely reduced perchlorate in the presence of cyanide. It was concluded that the inhibition of dissimilative perchlorate reduction by cyanide at this concentration was a consequence of oxygen accumulation, not inhibition of the enzymes used for perchlorate reduction. This finding on the effect of cyanide on respiratory enzymes provides a new method to control and study respiratory enzymes used for perchlorate reduction.

UR - http://www.scopus.com/inward/record.url?scp=5144235103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=5144235103&partnerID=8YFLogxK

U2 - 10.1016/j.femsle.2004.08.031

DO - 10.1016/j.femsle.2004.08.031

M3 - Article

C2 - 15476970

AN - SCOPUS:5144235103

VL - 239

SP - 229

EP - 234

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -