Inhibition of biohydrogen production by ammonia

Michael B. Salerno, Wooshin Park, Yi Zuo, Bruce Ernest Logan

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Ammonia inhibition of biohydrogen production was investigated in batch and continuous flow reactors with glucose as a substrate. In batch tests, biohydrogen production rate was highly dependent on pH and ammonia (defined as the sum of NH3 of NH4 + species) concentrations above 2 g N/L. At pH=6.2, the maximum production decreased from 56 mL/h at 2 g N/L to 16 mL/h at 10 g N/L. At pH=5.2, production decreased from 49 mL/h (2 g N/L) to 7 mL/h (16 g N/L). Hydrogen yield remained relatively constant in batch tests, varying from 0.96 to 1.17 mol-H2/mol-glucose. In continuous flow tests, both hydrogen production rates and yields were adversely affected by ammonia. When the reactor (2.0 L) was first acclimated under batch conditions to a low nitrogen concentration (<0.8 g N/L), H2 production and yields under continuous flow mode conditions were 170 mL/h and 1.9 mol-H 2/mol-glucose, but decreased with increased ammonia concentrations up to 7.8 g N/L to 105 mL/h and 1.1 mol-H2/mol-glucose. There was no hydrogen production under continuous flow conditions if the reactor was initially operated under batch flow conditions at ammonia concentrations above 0.8 g N/L. It is concluded that the hydrogen production is possible at high concentrations (up to 7.8 g N/L) of ammonia in continuous flow systems as long as the reactor is initially acclimated to a lower ammonia concentration (<0.8 g N/L).

Original languageEnglish (US)
Pages (from-to)1167-1172
Number of pages6
JournalWater Research
Volume40
Issue number6
DOIs
StatePublished - Mar 1 2006

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Ammonia
ammonia
Glucose
glucose
Hydrogen production
hydrogen
Nitrogen
substrate
Hydrogen
reactor
nitrogen
Substrates
test

All Science Journal Classification (ASJC) codes

  • Earth-Surface Processes

Cite this

Salerno, Michael B. ; Park, Wooshin ; Zuo, Yi ; Logan, Bruce Ernest. / Inhibition of biohydrogen production by ammonia. In: Water Research. 2006 ; Vol. 40, No. 6. pp. 1167-1172.
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Inhibition of biohydrogen production by ammonia. / Salerno, Michael B.; Park, Wooshin; Zuo, Yi; Logan, Bruce Ernest.

In: Water Research, Vol. 40, No. 6, 01.03.2006, p. 1167-1172.

Research output: Contribution to journalArticle

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AU - Salerno, Michael B.

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AU - Zuo, Yi

AU - Logan, Bruce Ernest

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