Inhibition of G1 to S phase progression by a novel zinc finger protein P58TFL at P-bodies

Kentaro Minagawa, Yoshio Katayama, Shinichiro Nishikawa, Katsuya Yamamoto, Akiko Sada, Atsuo Okamura, Manabu Shimoyama, Toshimitsu Matsui

Research output: Contribution to journalArticle

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Abstract

We recently reported the translocation of the immunoglobulin (Ig) light chain κ locus gene with a possible tumor suppressor gene, TFL, in transformed follicular lymphoma. However, the functional significance in cell transformation remains to be elucidated. Here, we first identified two gene products, P58TFL and P36TFL, derived by alternative splicing. The expression was prominent in normal human lymphocytes but defective in some leukemia/lymphoma cell lines. Overexpression of either protein in a mouse pro-B cell line, Ba/F3, and a human leukemia cell line, Jurkat, inhibited G1 to S phase progression through suppression of retinoblastoma protein (Rb) phosphorylation. The dominant gene product, P58TFL, colocalized with mRNA-processing body markers, eukaryotic translation initiation factor 2C and DCP1 decapping-enzyme homolog A, but not with a stress granule maker, T-cell intracellular antigen 1, in the cytoplasm. Taken together with the unique CCCH-type zinc finger motif, the present study suggests that P58 TFL could play an important role in the regulation of cell growth through posttranscriptional modification of cell cycle regulators, at least partially, upstream of Rb.

Original languageEnglish (US)
Pages (from-to)880-889
Number of pages10
JournalMolecular Cancer Research
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 1 2009

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Oncology
  • Cancer Research

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