Inhibition of hemagglutination activity of influenza a viruses by SP-A1 and SP-A2 variants expressed in CHO cells

Anatoly N. Mikerov, Mitch White, Kevan Hartshorn, Guirong Wang, Joanna Floros

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Surfactant protein A (SP-A) inhibits hemagglutination (HA) activity and infectivity of influenza A viruses (IAV). As we have showed before in different assays, SP-A2 gene products are more active than SP-A1. Here, we hypothesized that SP-A1 and SP-A2 mammalian CHO-cell-expressed proteins also differentially modulate HA inhibition of IAV. We found that both SP-A1 and SP-A2 equally displayed α(2,3)-linked sialic acids, and had similar activity against a strain (PR-8) that preferentially binds to α(2,3)-linked sialic acids. Based on these findings, we speculate that in human lung SP-A1 and SP-A2 will not be different in their activity against IAV that preferably bind to α(2,3)-linked sialic acids (like avian strains).

Original languageEnglish (US)
Pages (from-to)9-12
Number of pages4
JournalMedical Microbiology and Immunology
Volume197
Issue number1
DOIs
StatePublished - Mar 1 2008

Fingerprint

CHO Cells
Hemagglutination
Sialic Acids
Orthomyxoviridae
varespladib methyl
Influenza A virus
Pulmonary Surfactant-Associated Protein A
Lung
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

Cite this

Mikerov, Anatoly N. ; White, Mitch ; Hartshorn, Kevan ; Wang, Guirong ; Floros, Joanna. / Inhibition of hemagglutination activity of influenza a viruses by SP-A1 and SP-A2 variants expressed in CHO cells. In: Medical Microbiology and Immunology. 2008 ; Vol. 197, No. 1. pp. 9-12.
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Inhibition of hemagglutination activity of influenza a viruses by SP-A1 and SP-A2 variants expressed in CHO cells. / Mikerov, Anatoly N.; White, Mitch; Hartshorn, Kevan; Wang, Guirong; Floros, Joanna.

In: Medical Microbiology and Immunology, Vol. 197, No. 1, 01.03.2008, p. 9-12.

Research output: Contribution to journalArticle

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