Inhibition of hemagglutination activity of influenza a viruses by SP-A1 and SP-A2 variants expressed in CHO cells

Anatoly N. Mikerov, Mitch White, Kevan Hartshorn, Guirong Wang, Joanna Floros

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Surfactant protein A (SP-A) inhibits hemagglutination (HA) activity and infectivity of influenza A viruses (IAV). As we have showed before in different assays, SP-A2 gene products are more active than SP-A1. Here, we hypothesized that SP-A1 and SP-A2 mammalian CHO-cell-expressed proteins also differentially modulate HA inhibition of IAV. We found that both SP-A1 and SP-A2 equally displayed α(2,3)-linked sialic acids, and had similar activity against a strain (PR-8) that preferentially binds to α(2,3)-linked sialic acids. Based on these findings, we speculate that in human lung SP-A1 and SP-A2 will not be different in their activity against IAV that preferably bind to α(2,3)-linked sialic acids (like avian strains).

Original languageEnglish (US)
Pages (from-to)9-12
Number of pages4
JournalMedical Microbiology and Immunology
Volume197
Issue number1
DOIs
StatePublished - Mar 2008

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

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