Inhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites

Structure-activity relationship and molecular-modeling studies

Dapeng Chen, Ching Y. Wang, Joshua D. Lambert, Ni Ai, William J. Welsh, Chung S. Yang

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

(-)-Epigallocatechin-3-gallate (EGCG) is the major polyphenol present in green tea. We previously demonstrated that EGCG was both a substrate and potent inhibitor of human liver cytosolic catechol-O-methyltransferease (COMT). We now report the structure-activity relationship for the inhibition of COMT-catalyzed O-methylation of catecholestrogens in human liver cytosol by tea catechins and some of their metabolites. The most potent inhibitors were catechins with a galloyl-type D-ring, including EGCG (IC50 = 0.07 μM), 4″-O-methyl-EGCG (IC50 = 0.10 μM), 4′,4″-di-O- methyl-EGCG (4′,4″-DiMeEGCG) (IC50 = 0.15 μM), and (-)-epicatechin-3-gallate (ECG) (IC50 = 0.20 μM). Catechins without the D-ring showed two to three orders of magnitude less inhibitory potency. Enzyme kinetic analyses revealed that EGCG behaved as a mixed inhibitor, whereas 4′,4″-di-O-methyl-EGCG exhibited competitive kinetics for the S-adenosylmethionine (SAM), and noncompetitive kinetics for the catechol binding site. These compounds may represent a new type of COMT inhibitor. In silico molecular-modeling studies using a homology model of human COMT were conducted to aid in the understanding the catalytic and inhibitory mechanisms. Either D-ring or B-ring of EGCG could be accommodated to the substrate binding pocket of human COMT. However, the close proximity (2.6 Å) of 4″-OH to the critical residue Lys144, the higher acidity of the hydroxyl groups of the D-ring, and the hydrophobic interactions between the D-ring and residues in the binding pocket greatly facilitated the interaction of the D-ring with the enzyme, and resulted in increased inhibitory potency. These results provide mechanistic insight into the inhibition of COMT by commonly consumed tea catechins.

Original languageEnglish (US)
Pages (from-to)1523-1531
Number of pages9
JournalBiochemical Pharmacology
Volume69
Issue number10
DOIs
StatePublished - May 15 2005

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Catechol O-Methyltransferase
Molecular modeling
Catechin
Tea
Structure-Activity Relationship
Metabolites
Liver
Inhibitory Concentration 50
Catechol Estrogens
S-Adenosylmethionine
Enzyme kinetics
Methylation
epigallocatechin gallate
Polyphenols
Substrates
Enzymes
catechol
Hydrophobic and Hydrophilic Interactions
Acidity
Hydroxyl Radical

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology

Cite this

@article{648b065387cd4509aadcd4cea1b18ac1,
title = "Inhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites: Structure-activity relationship and molecular-modeling studies",
abstract = "(-)-Epigallocatechin-3-gallate (EGCG) is the major polyphenol present in green tea. We previously demonstrated that EGCG was both a substrate and potent inhibitor of human liver cytosolic catechol-O-methyltransferease (COMT). We now report the structure-activity relationship for the inhibition of COMT-catalyzed O-methylation of catecholestrogens in human liver cytosol by tea catechins and some of their metabolites. The most potent inhibitors were catechins with a galloyl-type D-ring, including EGCG (IC50 = 0.07 μM), 4″-O-methyl-EGCG (IC50 = 0.10 μM), 4′,4″-di-O- methyl-EGCG (4′,4″-DiMeEGCG) (IC50 = 0.15 μM), and (-)-epicatechin-3-gallate (ECG) (IC50 = 0.20 μM). Catechins without the D-ring showed two to three orders of magnitude less inhibitory potency. Enzyme kinetic analyses revealed that EGCG behaved as a mixed inhibitor, whereas 4′,4″-di-O-methyl-EGCG exhibited competitive kinetics for the S-adenosylmethionine (SAM), and noncompetitive kinetics for the catechol binding site. These compounds may represent a new type of COMT inhibitor. In silico molecular-modeling studies using a homology model of human COMT were conducted to aid in the understanding the catalytic and inhibitory mechanisms. Either D-ring or B-ring of EGCG could be accommodated to the substrate binding pocket of human COMT. However, the close proximity (2.6 {\AA}) of 4″-OH to the critical residue Lys144, the higher acidity of the hydroxyl groups of the D-ring, and the hydrophobic interactions between the D-ring and residues in the binding pocket greatly facilitated the interaction of the D-ring with the enzyme, and resulted in increased inhibitory potency. These results provide mechanistic insight into the inhibition of COMT by commonly consumed tea catechins.",
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Inhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites : Structure-activity relationship and molecular-modeling studies. / Chen, Dapeng; Wang, Ching Y.; Lambert, Joshua D.; Ai, Ni; Welsh, William J.; Yang, Chung S.

In: Biochemical Pharmacology, Vol. 69, No. 10, 15.05.2005, p. 1523-1531.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites

T2 - Structure-activity relationship and molecular-modeling studies

AU - Chen, Dapeng

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N2 - (-)-Epigallocatechin-3-gallate (EGCG) is the major polyphenol present in green tea. We previously demonstrated that EGCG was both a substrate and potent inhibitor of human liver cytosolic catechol-O-methyltransferease (COMT). We now report the structure-activity relationship for the inhibition of COMT-catalyzed O-methylation of catecholestrogens in human liver cytosol by tea catechins and some of their metabolites. The most potent inhibitors were catechins with a galloyl-type D-ring, including EGCG (IC50 = 0.07 μM), 4″-O-methyl-EGCG (IC50 = 0.10 μM), 4′,4″-di-O- methyl-EGCG (4′,4″-DiMeEGCG) (IC50 = 0.15 μM), and (-)-epicatechin-3-gallate (ECG) (IC50 = 0.20 μM). Catechins without the D-ring showed two to three orders of magnitude less inhibitory potency. Enzyme kinetic analyses revealed that EGCG behaved as a mixed inhibitor, whereas 4′,4″-di-O-methyl-EGCG exhibited competitive kinetics for the S-adenosylmethionine (SAM), and noncompetitive kinetics for the catechol binding site. These compounds may represent a new type of COMT inhibitor. In silico molecular-modeling studies using a homology model of human COMT were conducted to aid in the understanding the catalytic and inhibitory mechanisms. Either D-ring or B-ring of EGCG could be accommodated to the substrate binding pocket of human COMT. However, the close proximity (2.6 Å) of 4″-OH to the critical residue Lys144, the higher acidity of the hydroxyl groups of the D-ring, and the hydrophobic interactions between the D-ring and residues in the binding pocket greatly facilitated the interaction of the D-ring with the enzyme, and resulted in increased inhibitory potency. These results provide mechanistic insight into the inhibition of COMT by commonly consumed tea catechins.

AB - (-)-Epigallocatechin-3-gallate (EGCG) is the major polyphenol present in green tea. We previously demonstrated that EGCG was both a substrate and potent inhibitor of human liver cytosolic catechol-O-methyltransferease (COMT). We now report the structure-activity relationship for the inhibition of COMT-catalyzed O-methylation of catecholestrogens in human liver cytosol by tea catechins and some of their metabolites. The most potent inhibitors were catechins with a galloyl-type D-ring, including EGCG (IC50 = 0.07 μM), 4″-O-methyl-EGCG (IC50 = 0.10 μM), 4′,4″-di-O- methyl-EGCG (4′,4″-DiMeEGCG) (IC50 = 0.15 μM), and (-)-epicatechin-3-gallate (ECG) (IC50 = 0.20 μM). Catechins without the D-ring showed two to three orders of magnitude less inhibitory potency. Enzyme kinetic analyses revealed that EGCG behaved as a mixed inhibitor, whereas 4′,4″-di-O-methyl-EGCG exhibited competitive kinetics for the S-adenosylmethionine (SAM), and noncompetitive kinetics for the catechol binding site. These compounds may represent a new type of COMT inhibitor. In silico molecular-modeling studies using a homology model of human COMT were conducted to aid in the understanding the catalytic and inhibitory mechanisms. Either D-ring or B-ring of EGCG could be accommodated to the substrate binding pocket of human COMT. However, the close proximity (2.6 Å) of 4″-OH to the critical residue Lys144, the higher acidity of the hydroxyl groups of the D-ring, and the hydrophobic interactions between the D-ring and residues in the binding pocket greatly facilitated the interaction of the D-ring with the enzyme, and resulted in increased inhibitory potency. These results provide mechanistic insight into the inhibition of COMT by commonly consumed tea catechins.

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