Inhibition of hydrogen uptake in escherichia coli by expressing the hydrogenase from the Cyanobacterium Synechocystis Sp. Pcc 6803

Toshinari Maeda, Gönül Vardar, William T. Self, Thomas K. Wood

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 as well as the native Escherichia coli hydrogenase 3 hold great promise for hydrogen generation. The reversible (bi-directional) hydrogenase (hoxEFUYH) of Synechocystis sp. PCC 6803 is a [NiFe]-type enzyme that produces hydrogen via the reaction 2H+ + 2e- ↔ H2 (g); the source of the two electrons is NADH. Hydrogenase enzymes in E. coli are involved in two distinct modes of hydrogen metabolism: hydrogen production via hydrogenase 3 (encoded by hycABCDEFGHI) and hydrogen uptake by hydrogenase 1 (encoded by hyaABCDEF) and hydrogenase 2 (encoded by hybOABCDEFG). A study was conducted to determine how the cyanobacterial locus increases hydrogen production. Different components of the cyanobacterial locus were expressed. Overexpression of HoxU alone (small diaphorase subunit) accounted for 43% of the additional hydrogen produced by HoxEFUYH. Hydrogen production in TG1/pBS(Kan)HoxUYH was somewhat better than that in TG1/pBS(Kan)Synhox indicating the importance of proteins HoxU, HoxY, and HoxH. This is an abstract of a paper presented at the AIChE Annual Meeting (Salt Lake City, UT 11/4-9/2007).

Original languageEnglish (US)
Title of host publication2007 AIChE Annual Meeting
StatePublished - Dec 1 2007
Event2007 AIChE Annual Meeting - Salt Lake City, UT, United States
Duration: Nov 4 2007Nov 9 2007

Publication series

Name2007 AIChE Annual Meeting

Other

Other2007 AIChE Annual Meeting
CountryUnited States
CitySalt Lake City, UT
Period11/4/0711/9/07

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Chemical Engineering(all)
  • Bioengineering
  • Safety, Risk, Reliability and Quality

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    Maeda, T., Vardar, G., Self, W. T., & Wood, T. K. (2007). Inhibition of hydrogen uptake in escherichia coli by expressing the hydrogenase from the Cyanobacterium Synechocystis Sp. Pcc 6803. In 2007 AIChE Annual Meeting (2007 AIChE Annual Meeting).