Inhibition of key digestive enzymes by cocoa extracts and procyanidins

Yeyi Gu, William J. Hurst, David A. Stuart, Joshua D. Lambert

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

This study determined the in vitro inhibitory effects of cocoa extracts and procyanidins against pancreatic a-amylase (PA), pancreatic lipase (PL), and secreted phospholipase A2 (PLA2) and characterized the kinetics of such inhibition. Lavado, regular, and Dutch-processed cocoa extracts as well as cocoa procyanidins (degree of polymerization (DP) = 2-10) were examined. Cocoa extracts and procyanidins dose-dependently inhibited PA, PL, and PLA2. Lavado cocoa extract was the most potent inhibitor (IC 50 = 8.5-47μg/mL). An inverse correlation between log IC 50 and DP (R2 < 0.93) was observed. Kinetic analysis suggested that regular cocoa extract, the pentamer, and decamer inhibited PL activity in a mixed mode. The pentamer and decamer noncompetitively inhibited PLA2 activity, whereas regular cocoa extract inhibited PLA 2 competitively. This study demonstrates that cocoa polyphenols can inhibit digestive enzymes in vitro and may, in conjunction with a low-calorie diet, play a role in body weight management.

Original languageEnglish (US)
Pages (from-to)5305-5311
Number of pages7
JournalJournal of agricultural and food chemistry
Volume59
Issue number10
DOIs
StatePublished - May 25 2011

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Cocoa
Proanthocyanidins
procyanidins
digestive enzymes
Lipase
Phospholipases A2
Amylases
Polymerization
phospholipase A2
extracts
Enzymes
Secretory Phospholipase A2
triacylglycerol lipase
Caloric Restriction
Polyphenols
amylases
polymerization
inhibitory concentration 50
Body Weight
kinetics

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Gu, Yeyi ; Hurst, William J. ; Stuart, David A. ; Lambert, Joshua D. / Inhibition of key digestive enzymes by cocoa extracts and procyanidins. In: Journal of agricultural and food chemistry. 2011 ; Vol. 59, No. 10. pp. 5305-5311.
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Inhibition of key digestive enzymes by cocoa extracts and procyanidins. / Gu, Yeyi; Hurst, William J.; Stuart, David A.; Lambert, Joshua D.

In: Journal of agricultural and food chemistry, Vol. 59, No. 10, 25.05.2011, p. 5305-5311.

Research output: Contribution to journalArticle

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