Inhibition of migration and invasion in melanoma cells by β-escin via the ERK/NF-κB signaling pathway

Hyeong Seob Kwak, Hongyan An, Md Badrul Alam, Won Sik Choi, Sang Lee, Sang Han Lee

Research output: Contribution to journalArticle

Abstract

β-Escin, a natural triterpene saponin was extracted from Aesculus hippocastanum seeds, which have been widely used to treat inflammation in traditional medicine. In an effort to study the possible anti-tumor effects of β-escin, we performed wound healing, invasion, and adhesion assays to examine the effects of β-escin on cell migration, invasion, and angiogenesis. Our results revealed that β-escin inhibits cell migration as well as motility in B16F10 and SK-MEL5 cells in a dose-dependent manner. RT-PCR and Western blot analysis showed that β-escin increased TIMP-1, -2 while significantly downregulated phosphorylated extracellular signal-regulated kinase (p-ERK) expression, and suppressing nuclear factor-kappa B (NF-κB) and inhibitor of nuclear factor-kappa B (IκB) expression. Overall, the data from the current study suggest that β-escin has the potential for inhibiting both metastatic and angiogenic activities, and are the earliest evidence for the involvement of the NF-κB/IκB signaling in β-escin-induced anti-tumor effects.

Original languageEnglish (US)
Pages (from-to)1606-1610
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume41
Issue number10
DOIs
StatePublished - Jan 1 2018

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Escin
NF-kappa B
Melanoma
Cell Movement
Aesculus
Tissue Inhibitor of Metalloproteinase-2
Triterpenes
Tissue Inhibitor of Metalloproteinase-1
Extracellular Signal-Regulated MAP Kinases
Saponins
Traditional Medicine
Wound Healing
Neoplasms
Seeds
Down-Regulation
Western Blotting
Inflammation
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Kwak, Hyeong Seob ; An, Hongyan ; Alam, Md Badrul ; Choi, Won Sik ; Lee, Sang ; Lee, Sang Han. / Inhibition of migration and invasion in melanoma cells by β-escin via the ERK/NF-κB signaling pathway. In: Biological and Pharmaceutical Bulletin. 2018 ; Vol. 41, No. 10. pp. 1606-1610.
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Inhibition of migration and invasion in melanoma cells by β-escin via the ERK/NF-κB signaling pathway. / Kwak, Hyeong Seob; An, Hongyan; Alam, Md Badrul; Choi, Won Sik; Lee, Sang; Lee, Sang Han.

In: Biological and Pharmaceutical Bulletin, Vol. 41, No. 10, 01.01.2018, p. 1606-1610.

Research output: Contribution to journalArticle

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AU - Kwak, Hyeong Seob

AU - An, Hongyan

AU - Alam, Md Badrul

AU - Choi, Won Sik

AU - Lee, Sang

AU - Lee, Sang Han

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