Inhibition of O6-alkylguanine-DNA-alkyltransferase by metals

David A. Scicchitano, Anthony Pegg

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The activity of the DNA-repair protein O6-alkylguanine-DNA-alkyltransferase was found to be strongly inhibited by a number of metal ions. Cd2+ was the most active followed by Cu2+, Hg2+, Zn2+ and Ag2. This inhibition is likely to result from the interaction of the metals with the cysteine-acceptor residue on the protein since the inhibition was reduced by increasing the concentration of dithiothreitol in the assay buffer. These results raise the possibility that exposure to Cd2+ could increase the mutagenicity and carcinogenicity of alkylating agents by retarding the rate of repair of alkylated DNA. However, other metals or metallic compounds which are known to be carcinogenic (such as compounds containing arsenic, lead, nickel or chromium) did not interfere with DNA repair by this protein.

Original languageEnglish (US)
Pages (from-to)207-210
Number of pages4
JournalMutation Research Letters
Volume192
Issue number3
DOIs
StatePublished - Jan 1 1987

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DNA Repair
Metals
Arsenicals
Proteins
Dithiothreitol
Alkylating Agents
Chromium
Nickel
Cysteine
Buffers
Ions
DNA alkyltransferase
Lead

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Scicchitano, David A. ; Pegg, Anthony. / Inhibition of O6-alkylguanine-DNA-alkyltransferase by metals. In: Mutation Research Letters. 1987 ; Vol. 192, No. 3. pp. 207-210.
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Inhibition of O6-alkylguanine-DNA-alkyltransferase by metals. / Scicchitano, David A.; Pegg, Anthony.

In: Mutation Research Letters, Vol. 192, No. 3, 01.01.1987, p. 207-210.

Research output: Contribution to journalArticle

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