Inhibition of TATA binding protein dimerization by RNA polymerase III transcription initiation factor Brf1

Diane E. Alexander, David J. Kaczorowski, Amy J. Jackson-Fisher, Drew M. Lowery, Sara J. Zanton, Benjamin Franklin Pugh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Brf1 subunit of TFIIIB plays an important role in recruiting the TATA-binding protein (TBP) to the up-stream region of genes transcribed by RNA polymerase III. When TBP is not bound to promoters, it sequesters its DNA binding domain through dimerization. Promoter assembly factors therefore might be required to dissociate TBP into productively binding monomers. Here we show that Saccharomyces cerevisiae Brf1 induces TBP dimers to dissociate. The high affinity TBP binding domain of Brf1 is not sufficient to promote TBP dimer dissociation but in addition requires the TFIIB homology domain of Brf1. A model is proposed to explain how two distinct functional domains of Brf1 work in concert to dissociate TBP into monomers.

Original languageEnglish (US)
Pages (from-to)32401-32406
Number of pages6
JournalJournal of Biological Chemistry
Volume279
Issue number31
DOIs
StatePublished - Jul 30 2004

Fingerprint

Protein Multimerization
TATA-Box Binding Protein
RNA Polymerase III
Peptide Initiation Factors
Dimerization
Transcription
Transcription Factors
Dimers
Transcription Factor TFIIIB
Monomers
Transcription Factor TFIIB
Protein Binding
Yeast
Saccharomyces cerevisiae
Genes
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Alexander, Diane E. ; Kaczorowski, David J. ; Jackson-Fisher, Amy J. ; Lowery, Drew M. ; Zanton, Sara J. ; Pugh, Benjamin Franklin. / Inhibition of TATA binding protein dimerization by RNA polymerase III transcription initiation factor Brf1. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 31. pp. 32401-32406.
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Inhibition of TATA binding protein dimerization by RNA polymerase III transcription initiation factor Brf1. / Alexander, Diane E.; Kaczorowski, David J.; Jackson-Fisher, Amy J.; Lowery, Drew M.; Zanton, Sara J.; Pugh, Benjamin Franklin.

In: Journal of Biological Chemistry, Vol. 279, No. 31, 30.07.2004, p. 32401-32406.

Research output: Contribution to journalArticle

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AU - Alexander, Diane E.

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