TY - JOUR
T1 - Inhibition of the mixed lymphocyte proliferative response by phorbol esters
AU - Mastro, Andrea M.
AU - Mueller, Gerald C.
N1 - Funding Information:
This study was supported by U.S. Public Health Service Grants T01-CA-5002 and CA-07175 to G.C.M., and in part by a grant from NIH (GM 22953}
Funding Information:
to A.M.M.G.C.M. is the recipient of a Research Career Development Award, U.S.A. Public Health Service. A.M.M. was a Damon Runyon Postdoctoral Fellow. The authors thank Dr. Tucker Helmes for his gift of the phorbol esters and Ms. Mary LeMahieu for her help with preparation of this manuscript.
PY - 1978/1/26
Y1 - 1978/1/26
N2 - The phorbol diester, 12-O-tetradecanoyl-phorbol-13-acetate, a potent cocarcinogen in mice, blocks the induction of DNA synthesis in lymphocytes undergoing the mixed lymphocyte response. At 10-7 M diester, the induced DNA synthesis is inhibited almost completely (99%). This action of the diester affects some early step in the response which is necessary for the triggering of cell replication; on-going DNA replication is not significantly affected. Phorbol 12,13-diacetate, a less potent analogue in tumor promotion in vivo, is also a less potent inhibitor of the mixed lymphocyte response (75% inhibition at 10-6 M). Phorbol, the parent alcohol, is not effective in either system. The use of phorbol diesters in the molecular dissection of mixed lymphocyte responses is discussed.
AB - The phorbol diester, 12-O-tetradecanoyl-phorbol-13-acetate, a potent cocarcinogen in mice, blocks the induction of DNA synthesis in lymphocytes undergoing the mixed lymphocyte response. At 10-7 M diester, the induced DNA synthesis is inhibited almost completely (99%). This action of the diester affects some early step in the response which is necessary for the triggering of cell replication; on-going DNA replication is not significantly affected. Phorbol 12,13-diacetate, a less potent analogue in tumor promotion in vivo, is also a less potent inhibitor of the mixed lymphocyte response (75% inhibition at 10-6 M). Phorbol, the parent alcohol, is not effective in either system. The use of phorbol diesters in the molecular dissection of mixed lymphocyte responses is discussed.
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U2 - 10.1016/0005-2787(78)90052-7
DO - 10.1016/0005-2787(78)90052-7
M3 - Article
C2 - 146519
AN - SCOPUS:0017798386
SN - 0005-2787
VL - 517
SP - 246
EP - 254
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -