Inhibition of the mixed lymphocyte proliferative response by phorbol esters

Andrea M. Mastro, Gerald C. Mueller

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Abstract

The phorbol diester, 12-O-tetradecanoyl-phorbol-13-acetate, a potent cocarcinogen in mice, blocks the induction of DNA synthesis in lymphocytes undergoing the mixed lymphocyte response. At 10-7 M diester, the induced DNA synthesis is inhibited almost completely (99%). This action of the diester affects some early step in the response which is necessary for the triggering of cell replication; on-going DNA replication is not significantly affected. Phorbol 12,13-diacetate, a less potent analogue in tumor promotion in vivo, is also a less potent inhibitor of the mixed lymphocyte response (75% inhibition at 10-6 M). Phorbol, the parent alcohol, is not effective in either system. The use of phorbol diesters in the molecular dissection of mixed lymphocyte responses is discussed.

Original languageEnglish (US)
Pages (from-to)246-254
Number of pages9
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume517
Issue number1
DOIs
StatePublished - Jan 26 1978

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

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