Inhibition of tumor necrosis factor in the brain suppresses rabbit sleep

Satoshi Takahashi, Dawn D. Tooley, Levente Kapás, Jidong Fang, Jerome M. Seyer, James M. Krueger

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Tumor necrosis factor (TNF) is a cytokine that possesses many biological activities, including enhancement of non-rapid-eye-movement sleep (NREMS). The role of endogenous TNF in the regulation of spontaneous sleep is unknown. If TNF is involved in sleep regulation, then reduction of endogenous TNF should suppress spontaneous sleep. A soluble TNF-binding protein I (TNF-BP I) and a synthetic fragment of TNF-BP I, TNF-R-(159-178), that contains the biologically active region of TNF-BP I, were used. These substances bind TNF and possess TNF-inhibitory activity; their effects on rabbit sleep after intracerebroventricular injection were determined across a 6-h recording period. Two doses of TNF-BP I (0.05 μg and 0.5 μg) were administered; the higher dose of TNF-BP I significantly decreased NREMS. Four doses of TNF-R-(159-178) (0.25 μg, 2.5 μg, 25 μg and 50 μg) were used. The 25 μg and 50 μg doses significantly suppressed NREMS. The highest dose (50 μg) also decreased REM sleep. These results are consistent with the hypothesis that endogenous brain TNF is involved in the regulation of normal sleep.

Original languageEnglish (US)
Pages (from-to)155-160
Number of pages6
JournalPflügers Archiv European Journal of Physiology
Volume431
Issue number2
DOIs
StatePublished - Dec 1 1995

Fingerprint

Brain
Sleep
Tumor Necrosis Factor-alpha
Rabbits
Eye movements
Eye Movements
Inhibition (Psychology)
REM Sleep
Bioactivity
Carrier Proteins
Cytokines
Injections

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Takahashi, Satoshi ; Tooley, Dawn D. ; Kapás, Levente ; Fang, Jidong ; Seyer, Jerome M. ; Krueger, James M. / Inhibition of tumor necrosis factor in the brain suppresses rabbit sleep. In: Pflügers Archiv European Journal of Physiology. 1995 ; Vol. 431, No. 2. pp. 155-160.
@article{451b40212cda490bb4ea2cfb00ba75c5,
title = "Inhibition of tumor necrosis factor in the brain suppresses rabbit sleep",
abstract = "Tumor necrosis factor (TNF) is a cytokine that possesses many biological activities, including enhancement of non-rapid-eye-movement sleep (NREMS). The role of endogenous TNF in the regulation of spontaneous sleep is unknown. If TNF is involved in sleep regulation, then reduction of endogenous TNF should suppress spontaneous sleep. A soluble TNF-binding protein I (TNF-BP I) and a synthetic fragment of TNF-BP I, TNF-R-(159-178), that contains the biologically active region of TNF-BP I, were used. These substances bind TNF and possess TNF-inhibitory activity; their effects on rabbit sleep after intracerebroventricular injection were determined across a 6-h recording period. Two doses of TNF-BP I (0.05 μg and 0.5 μg) were administered; the higher dose of TNF-BP I significantly decreased NREMS. Four doses of TNF-R-(159-178) (0.25 μg, 2.5 μg, 25 μg and 50 μg) were used. The 25 μg and 50 μg doses significantly suppressed NREMS. The highest dose (50 μg) also decreased REM sleep. These results are consistent with the hypothesis that endogenous brain TNF is involved in the regulation of normal sleep.",
author = "Satoshi Takahashi and Tooley, {Dawn D.} and Levente Kap{\'a}s and Jidong Fang and Seyer, {Jerome M.} and Krueger, {James M.}",
year = "1995",
month = "12",
day = "1",
doi = "10.1007/BF00410186",
language = "English (US)",
volume = "431",
pages = "155--160",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "2",

}

Inhibition of tumor necrosis factor in the brain suppresses rabbit sleep. / Takahashi, Satoshi; Tooley, Dawn D.; Kapás, Levente; Fang, Jidong; Seyer, Jerome M.; Krueger, James M.

In: Pflügers Archiv European Journal of Physiology, Vol. 431, No. 2, 01.12.1995, p. 155-160.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibition of tumor necrosis factor in the brain suppresses rabbit sleep

AU - Takahashi, Satoshi

AU - Tooley, Dawn D.

AU - Kapás, Levente

AU - Fang, Jidong

AU - Seyer, Jerome M.

AU - Krueger, James M.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - Tumor necrosis factor (TNF) is a cytokine that possesses many biological activities, including enhancement of non-rapid-eye-movement sleep (NREMS). The role of endogenous TNF in the regulation of spontaneous sleep is unknown. If TNF is involved in sleep regulation, then reduction of endogenous TNF should suppress spontaneous sleep. A soluble TNF-binding protein I (TNF-BP I) and a synthetic fragment of TNF-BP I, TNF-R-(159-178), that contains the biologically active region of TNF-BP I, were used. These substances bind TNF and possess TNF-inhibitory activity; their effects on rabbit sleep after intracerebroventricular injection were determined across a 6-h recording period. Two doses of TNF-BP I (0.05 μg and 0.5 μg) were administered; the higher dose of TNF-BP I significantly decreased NREMS. Four doses of TNF-R-(159-178) (0.25 μg, 2.5 μg, 25 μg and 50 μg) were used. The 25 μg and 50 μg doses significantly suppressed NREMS. The highest dose (50 μg) also decreased REM sleep. These results are consistent with the hypothesis that endogenous brain TNF is involved in the regulation of normal sleep.

AB - Tumor necrosis factor (TNF) is a cytokine that possesses many biological activities, including enhancement of non-rapid-eye-movement sleep (NREMS). The role of endogenous TNF in the regulation of spontaneous sleep is unknown. If TNF is involved in sleep regulation, then reduction of endogenous TNF should suppress spontaneous sleep. A soluble TNF-binding protein I (TNF-BP I) and a synthetic fragment of TNF-BP I, TNF-R-(159-178), that contains the biologically active region of TNF-BP I, were used. These substances bind TNF and possess TNF-inhibitory activity; their effects on rabbit sleep after intracerebroventricular injection were determined across a 6-h recording period. Two doses of TNF-BP I (0.05 μg and 0.5 μg) were administered; the higher dose of TNF-BP I significantly decreased NREMS. Four doses of TNF-R-(159-178) (0.25 μg, 2.5 μg, 25 μg and 50 μg) were used. The 25 μg and 50 μg doses significantly suppressed NREMS. The highest dose (50 μg) also decreased REM sleep. These results are consistent with the hypothesis that endogenous brain TNF is involved in the regulation of normal sleep.

UR - http://www.scopus.com/inward/record.url?scp=0029615332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029615332&partnerID=8YFLogxK

U2 - 10.1007/BF00410186

DO - 10.1007/BF00410186

M3 - Article

C2 - 9026774

AN - SCOPUS:0029615332

VL - 431

SP - 155

EP - 160

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 2

ER -