Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

Reyad A. Elbarbary, Hiroaki Takaku, Masato Tamura, Masayuki Nashimoto

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Pathogenic angiogenesis in various diseases including cancer, autoimmune diseases, and age-related macular degeneration is thought to be regressed with anti-angiogenic drugs. TRUE gene silencing is a new technology to eliminate a specific mRNA using synthetic sgRNA and cellular tRNase ZL. To discover anti-angiogenic sgRNAs, we applied TRUE silencing to the VEGF gene. We examined eight sgRNAs for efficacy in targeting exogenous human VEGF mRNA. Many of them worked efficiently in 293 and HeLa cells. Two of them downregulated the endogenous VEGF gene expression in HeLa cells very efficiently, and the efficacy of these two sgRNAs surpassed that of siRNA extremely.

Original languageEnglish (US)
Pages (from-to)924-927
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume379
Issue number4
DOIs
Publication statusPublished - Feb 20 2009

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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