Inhibitory effect of antisense basic fibroblast growth factor oligonucleotides on proliferation of cultured aortic smooth muscle cells induced by angiotensin II in SHR rats

Guo Hong Li, Guo Jun Yang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

AIM: To study the effect of antisense basic fibroblast growth factor (bFGF) oligonucleotides (ODN) transfection on the growth of cultured aortic smooth muscle cells (SMC) in spontaneously hypertensive rats (SHR). METHODS: Using cationic liposome-mediated method, antisense bFGF ODN were introduced into SMC, bFGF gene expression was detected by Northern blotting, cell hyperplasia was evaluated by [3H ] thymidine incorporation and cell counting. RESULTS: Transfection of antisense bFGF ODN (5 μmol · L-1) almost completely inhibited enhanced bFGF mRNA expression and inhibited cell proliferation induced by angiotensin II (Ang 1 μmol · L-1). In basal state and Ang-stimulated state, [3H]thymidine incorporation was inhibited by 26.5 % (P<0.01) and 42.0 % (P<0.01) and cell number was inhibited by 17.3 % (P<0.01) and by 22.2 % (P < 0.01), respectively. CONCLUSION: The transfection of antisense bFGF ODN into cultured SMC effectively suppressed bFGF mRNA expression and inhibited the SMC proliferation induced by Ang.

Original languageEnglish (US)
Pages (from-to)132-135
Number of pages4
JournalActa Pharmacologica Sinica
Volume19
Issue number2
StatePublished - Mar 31 1998

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

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