Injection of fluorescently labeled analytes into microfabricated chips using optically gated electrophoresis

Julie A. Lapos, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Optically gated electrophoresis has been used as an alternative method of sample introduction in microfabricated chips. Utilization of this injection technique permits rapid serial sampling and consumes less chip space than traditional chip-based injection methods. 4-Chloro-7-nitrobenzofurazan (NBD)-labeled amino acids have been injected in microfabricated chips using optical gating and have yielded results comparable to the T-type injection methods currently used. Picoliter-size injection volumes have been reproducibly introduced with less than 3% deviation in retention time and peak area. Six consecutive separations have been accomplished in under 30 s using a separation length of 1.1 cm. Plate heights from this analysis range from 4.5 to 0.8 μm. The combination of rapid successive injections and reproducible separations demonstrates the utility of this method in analyzing rapid dynamic events such as on-line sampling or monitoring chemical processes.

Original languageEnglish (US)
Pages (from-to)4598-4602
Number of pages5
JournalAnalytical Chemistry
Volume72
Issue number19
DOIs
StatePublished - Oct 1 2000

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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