Inoculating Anopheles gambiae mosquitoes with beads to induce and measure the melanization immune response

Antoine M.G. Barreaux, Priscille Barreaux, Matthew B. Thomas, Jacob C. Koella

Research output: Contribution to journalArticle

Abstract

The stimulation of immune responses is a common tool in invertebrate studies to examine the efficacy and the mechanisms of immunity. This stimulation is based on the injection of non-pathogenic particles into insects, as the particles will be detected by the immune system and will induce the production of immune effectors. We focus here on the stimulation of the melanization response in the mosquito Anopheles gambiae. The melanization response results in the encapsulation of foreign particles and parasites with a dark layer of melanin. To stimulate this response, mosquitoes are inoculated with beads in the thoracic cavity using microcapillary glass tubes. Then, after 24 hr, the mosquitoes are dissected to retrieve the beads. The degree of melanization of the bead is measured using image analysis software. Beads do not have the pathogenic effects of parasites, or their capacity to evade or suppress the immune response. These injections are a way to measure immune efficacy and the impact of immune stimulations on other life history traits, such as fecundity or longevity. It is not exactly the same as directly studying host-parasite interactions, but it is an interesting tool to study immunity and its evolutionary ecology.

Original languageEnglish (US)
Article numbere55013
JournalJournal of Visualized Experiments
Volume2017
Issue number119
DOIs
StatePublished - Jan 2 2017

Fingerprint

Anopheles gambiae
Culicidae
Immunity
Parasites
Melanin
Thoracic Cavity
Host-Parasite Interactions
Injections
Immune system
Melanins
Ecology
Invertebrates
Encapsulation
Image analysis
Glass
Fertility
Insects
Immune System
Software

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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abstract = "The stimulation of immune responses is a common tool in invertebrate studies to examine the efficacy and the mechanisms of immunity. This stimulation is based on the injection of non-pathogenic particles into insects, as the particles will be detected by the immune system and will induce the production of immune effectors. We focus here on the stimulation of the melanization response in the mosquito Anopheles gambiae. The melanization response results in the encapsulation of foreign particles and parasites with a dark layer of melanin. To stimulate this response, mosquitoes are inoculated with beads in the thoracic cavity using microcapillary glass tubes. Then, after 24 hr, the mosquitoes are dissected to retrieve the beads. The degree of melanization of the bead is measured using image analysis software. Beads do not have the pathogenic effects of parasites, or their capacity to evade or suppress the immune response. These injections are a way to measure immune efficacy and the impact of immune stimulations on other life history traits, such as fecundity or longevity. It is not exactly the same as directly studying host-parasite interactions, but it is an interesting tool to study immunity and its evolutionary ecology.",
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Inoculating Anopheles gambiae mosquitoes with beads to induce and measure the melanization immune response. / Barreaux, Antoine M.G.; Barreaux, Priscille; Thomas, Matthew B.; Koella, Jacob C.

In: Journal of Visualized Experiments, Vol. 2017, No. 119, e55013, 02.01.2017.

Research output: Contribution to journalArticle

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