Inotropic response of rabbit ventricular myocytes to endothelin-1: Difference from isolated papillary muscles

M. A.Hassan Talukder, Ikuo Norota, Kiyoharu Sakurai, Masao Endoh

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Endothelin-1 (ET-1) increased cell shortening and Ca2+ transients over the concentration of 3 × 10-11 M to 10-9 M with EC50 of 8.3 × 10-11 M in rabbit single ventricular myocytes. Thus ET-1 was approximately 60 times more potent in single myocytes than in papillary muscles (EC50 = 5.1 × 10-9 M) of the same species. In single myocytes, ET-1 at 10-8 M elicited an inhibitory response that counteracted the facilitatory response: the concentration-response curve (CRC) for ET-1 was bell shaped. The ETA-receptor antagonist BQ-485 shifted CRC for ET-1 to the right in parallel; however, the facilitatory response to 10-8 M ET-1 was markedly enhanced by BQ-485 and also by the ETB antagonist BQ-788. The ETA/ETB antagonist TAK-044 abolished the ET-1-induced response. These findings indicate that the response to ET-1 of single myocytes is different from that of papillary muscles in concentration dependence, characteristics of the response, and susceptibility to ET-receptor antagonists. Anomalous pharmacological characteristics of ET-1-induced response in rabbit papillary muscles may be due to integrated regulatory mechanisms that may involve also various types of noncardiac cell in ventricular myocardium.

Original languageEnglish (US)
Pages (from-to)H596-H605
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume281
Issue number2 50-2
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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