Insecticidal and nematicidal properties of microbial metabolites

Saroj K. Mishra, James E. Keller, James R. Miller, Rod Martin Heisey, Muraleedharan G. Nair, Alan R. Putnam

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Metabolites from 942 microbial isolates were screened for insecticidal and nematicidal properties. The isolates included 302 streptomycetes, 502 'novel' actinomycetes including representatives of 18 genera, 28 unidentified aerobic actinomycetes, 70 fungi and 40 bacteria other than actinomycetes. When diluted 10-fold, the metabolites from 55 isolates caused nearly 100% mortality in mosquito larvae (Aedes aegypti) within 24 h. These isolates included 27 isolates of Streptomyces, four of Actinoplanes, three isolates each of Actinomadura and Streptoverticillium, two isolates each of Micromonospora, Bacillus and Paecilomyces, and one isolate each of Micropolyspora, Nocardiopsis, Streptosporangium, Oerskovia, Thermomonospora, Chainia, Pseudomonas, Fusarium, Monilia and Syncephalestrum. Two fungal isolates could not be identified to the generie level. Extracts from the culture broth of 18 isolates caused 100% mortality in mosquito larvae within 15 min to 24 h at a concentration of 1000 ppm. The LC50 of partially purified products from two isolates was 1-2.5 ppm and that of the semipurified preparations from four other isolates was ≤50 ppm. Valinomycin was identified as an active component in the culture broth from one isolate. The culture broth from 15 isolates of aerobic actinomycetes and 4 of fungi were toxic to Panagrellus redivivus (Nematoda); these included 12 isolates with selective nematicidal properties.

Original languageEnglish (US)
Pages (from-to)267-276
Number of pages10
JournalJournal of Industrial Microbiology
Volume2
Issue number5
DOIs
StatePublished - Dec 1 1987

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Actinobacteria
Metabolites
Fungi
Valinomycin
Poisons
Bacilli
Culicidae
Larva
Bacteria
Micromonospora
Paecilomyces
Nocardia
Nematoda
Mortality
Aedes
Fusarium
Streptomyces
Pseudomonas
Candida
Bacillus

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology

Cite this

Mishra, S. K., Keller, J. E., Miller, J. R., Heisey, R. M., Nair, M. G., & Putnam, A. R. (1987). Insecticidal and nematicidal properties of microbial metabolites. Journal of Industrial Microbiology, 2(5), 267-276. https://doi.org/10.1007/BF01569429
Mishra, Saroj K. ; Keller, James E. ; Miller, James R. ; Heisey, Rod Martin ; Nair, Muraleedharan G. ; Putnam, Alan R. / Insecticidal and nematicidal properties of microbial metabolites. In: Journal of Industrial Microbiology. 1987 ; Vol. 2, No. 5. pp. 267-276.
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Mishra, SK, Keller, JE, Miller, JR, Heisey, RM, Nair, MG & Putnam, AR 1987, 'Insecticidal and nematicidal properties of microbial metabolites', Journal of Industrial Microbiology, vol. 2, no. 5, pp. 267-276. https://doi.org/10.1007/BF01569429

Insecticidal and nematicidal properties of microbial metabolites. / Mishra, Saroj K.; Keller, James E.; Miller, James R.; Heisey, Rod Martin; Nair, Muraleedharan G.; Putnam, Alan R.

In: Journal of Industrial Microbiology, Vol. 2, No. 5, 01.12.1987, p. 267-276.

Research output: Contribution to journalArticle

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Mishra SK, Keller JE, Miller JR, Heisey RM, Nair MG, Putnam AR. Insecticidal and nematicidal properties of microbial metabolites. Journal of Industrial Microbiology. 1987 Dec 1;2(5):267-276. https://doi.org/10.1007/BF01569429