Insight into S-RNase-based self-incompatibility in Petunia: Recent findings and future directions

Justin S. Williams, Lihua Wu, Shu Li, Penglin Sun, Teh Hui Kao

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

S-RNase-based self-incompatibility in Petunia is a self/non-self recognition system that allows the pistil to reject self-pollen to prevent inbreeding and to accept non-self pollen for outcrossing. Cloning of S-RNase in 1986 marked the beginning of nearly three decades of intensive research into the mechanism of this complex system. S-RNase was shown to be the sole female determinant in 1994, and the first male determinant, S-locus F-box protein1 (SLF1), was identified in 2004. It was discovered in 2010 that additional SLF proteins are involved in pollen specificity, and recently two S-haplotypes of Petunia inflata were found to possess 17 SLF genes based on pollen transcriptome analysis, further increasing the complexity of the system. Here, we first summarize the current understanding of how the interplay between SLF proteins and S-RNase in the pollen tube allows cross-compatible pollination, but results in self-incompatible pollination. We then discuss some of the aspects that are not yet elucidated, including uptake of S-RNase into the pollen tube, nature, and assembly of SLF-containing complexes, the biochemical basis for differential interactions between SLF proteins and S-RNase, and fate of non-self S-RNases in the pollen tube.

Original languageEnglish (US)
Article number41
JournalFrontiers in Plant Science
Volume6
Issue numberFEB
DOIs
StatePublished - Feb 5 2015

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Petunia
pollen tubes
pollen
Petunia integrifolia
proteins
pistil
self-pollination
outcrossing
transcriptomics
inbreeding
molecular cloning
pollination
haplotypes
uptake mechanisms
loci
genes

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

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abstract = "S-RNase-based self-incompatibility in Petunia is a self/non-self recognition system that allows the pistil to reject self-pollen to prevent inbreeding and to accept non-self pollen for outcrossing. Cloning of S-RNase in 1986 marked the beginning of nearly three decades of intensive research into the mechanism of this complex system. S-RNase was shown to be the sole female determinant in 1994, and the first male determinant, S-locus F-box protein1 (SLF1), was identified in 2004. It was discovered in 2010 that additional SLF proteins are involved in pollen specificity, and recently two S-haplotypes of Petunia inflata were found to possess 17 SLF genes based on pollen transcriptome analysis, further increasing the complexity of the system. Here, we first summarize the current understanding of how the interplay between SLF proteins and S-RNase in the pollen tube allows cross-compatible pollination, but results in self-incompatible pollination. We then discuss some of the aspects that are not yet elucidated, including uptake of S-RNase into the pollen tube, nature, and assembly of SLF-containing complexes, the biochemical basis for differential interactions between SLF proteins and S-RNase, and fate of non-self S-RNases in the pollen tube.",
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Insight into S-RNase-based self-incompatibility in Petunia : Recent findings and future directions. / Williams, Justin S.; Wu, Lihua; Li, Shu; Sun, Penglin; Kao, Teh Hui.

In: Frontiers in Plant Science, Vol. 6, No. FEB, 41, 05.02.2015.

Research output: Contribution to journalArticle

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