Insights into okazaki fragment synthesis by the T4 replisome: The fate of lagging-strand holoenzyme components and their influence on okazaki fragment size

Danqi Chen, Hongjun Yue, Michelle M. Spiering, Stephen J. Benkovic

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Background: The T4 replisome duplicates the lagging DNA strand through discontinuous Okazaki fragments. Results: A third polymerase is not involved in repetitive Okazaki fragment synthesis. Conclusion: The T4 replisome recycles the lagging-strand polymerase but looses the clamp in each Okazaki fragment cycle through a collision or signaling pathway. Significance: Elucidation of the behavior of polymerase, clamp and clamp loader for Okazaki fragment synthesis broadens our understanding of coordinated DNA replication.

Original languageEnglish (US)
Pages (from-to)20807-20816
Number of pages10
JournalJournal of Biological Chemistry
Volume288
Issue number29
DOIs
StatePublished - Jul 19 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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