Insulin inhibits voltage-dependent calcium influx into rod photoreceptors

Salvatore Stella, E. J. Bryson, W. B. Thoreson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Insulin inhibits the ERG b-wave and modulates L-type calcium currents (ICa) in various preparations. We therefore examined insulin's effects on ICa and depolarization-evoked [Ca2+]i increases in rod photoreceptors. Insulin inhibited ICa and caused a dose-dependent reduction in the depolarization-evoked Ca2+ influx with an EC50 of 2.1 nM. Tyrosine kinase inhibitors, lavendustin A (100nM) and genistein (10μM), prevented insulin from reducing the depolarization-evoked Ca2+ increase in rods. Their less active analogues, lavendustin B and daidzein, had similar effects. An insulin receptor-specific tyrosine kinase inhibitor, HNMPA-(AM)3 (50μM), prevented insulin (30 nM) from reducing the depolarization-evoked Ca2+ increase in rods. The results suggest that insulin inhibits Ca2+ influx through voltage-dependent ICa in rod photoreceptors via tyrosine kinase activity.

Original languageEnglish (US)
Pages (from-to)947-951
Number of pages5
JournalNeuroReport
Volume12
Issue number5
StatePublished - Apr 17 2001

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Retinal Rod Photoreceptor Cells
Insulin
Calcium
Protein-Tyrosine Kinases
Genistein

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Stella, S., Bryson, E. J., & Thoreson, W. B. (2001). Insulin inhibits voltage-dependent calcium influx into rod photoreceptors. NeuroReport, 12(5), 947-951.
Stella, Salvatore ; Bryson, E. J. ; Thoreson, W. B. / Insulin inhibits voltage-dependent calcium influx into rod photoreceptors. In: NeuroReport. 2001 ; Vol. 12, No. 5. pp. 947-951.
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Stella, S, Bryson, EJ & Thoreson, WB 2001, 'Insulin inhibits voltage-dependent calcium influx into rod photoreceptors', NeuroReport, vol. 12, no. 5, pp. 947-951.

Insulin inhibits voltage-dependent calcium influx into rod photoreceptors. / Stella, Salvatore; Bryson, E. J.; Thoreson, W. B.

In: NeuroReport, Vol. 12, No. 5, 17.04.2001, p. 947-951.

Research output: Contribution to journalArticle

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AU - Stella, Salvatore

AU - Bryson, E. J.

AU - Thoreson, W. B.

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AB - Insulin inhibits the ERG b-wave and modulates L-type calcium currents (ICa) in various preparations. We therefore examined insulin's effects on ICa and depolarization-evoked [Ca2+]i increases in rod photoreceptors. Insulin inhibited ICa and caused a dose-dependent reduction in the depolarization-evoked Ca2+ influx with an EC50 of 2.1 nM. Tyrosine kinase inhibitors, lavendustin A (100nM) and genistein (10μM), prevented insulin from reducing the depolarization-evoked Ca2+ increase in rods. Their less active analogues, lavendustin B and daidzein, had similar effects. An insulin receptor-specific tyrosine kinase inhibitor, HNMPA-(AM)3 (50μM), prevented insulin (30 nM) from reducing the depolarization-evoked Ca2+ increase in rods. The results suggest that insulin inhibits Ca2+ influx through voltage-dependent ICa in rod photoreceptors via tyrosine kinase activity.

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