Insulin-like growth factor-1 treatment prevents anti-fas antibody-induced apoptosis in endplate chondrocytes

Yong Jun Wang, Qi Shi, Peng Sun, Quan Zhou, Michael Darowish, Tian Fang Li, Yu Feng Dong, William W. Lu, John C.Y. Leong

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Study Design. In vitro investigation of vertebral endplate chondrocyte apoptosis. Objectives. To determine whether Fas antibody caused apoptosis in endplate chondrocytes, and whether insulin-like growth factor-1 (IGF-1) inhibited this effect. Integrin-ā1 and focal adhesion kinase (FAK) expression in conjunction with apoptosis was also investigated. Summary of Background Data. Binding of Fas antibody to Fas mimics Fas-FasL ligation, which causes apoptosis. IGF-1 has been shown to have anti-apoptotic effects. Materials and Methods. Rat cervical endplate chondrocytes were cultured and treated with Fas antibody, with or without IGF-1. Cellular morphology was examined by microscopy. Apoptotic changes were evaluated by transmission electron microscopy, TUNEL staining, and immunostaining. Apoptosis-induced changes in the expression of integrin-ā1 chain and FAK were also investigated. Results. Endplate chondrocytes were able to be cultured; a chondrocytic phenotype was maintained. Fas antibody induced apoptosis in endplate chondrocytes; this was confirmed by TUNEL staining. Bcl-2 expression was decreased by Fas antibody, while Bax expression increased. Integrin-ā1 and FAK expression was decreased by Fas antibody. IGF-1 treatment inhibited these Fas antibody-induced changes. Conclusions. Fas antibody induces apoptosis and decreases Integrin-ā1 and FAK expression in cultured endplate chondrocytes; IGF-1 is protective against these changes.

Original languageEnglish (US)
Pages (from-to)736-741
Number of pages6
JournalSpine
Volume31
Issue number7
DOIs
StatePublished - Apr 1 2006

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine
  • Clinical Neurology

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    Wang, Y. J., Shi, Q., Sun, P., Zhou, Q., Darowish, M., Li, T. F., Dong, Y. F., Lu, W. W., & Leong, J. C. Y. (2006). Insulin-like growth factor-1 treatment prevents anti-fas antibody-induced apoptosis in endplate chondrocytes. Spine, 31(7), 736-741. https://doi.org/10.1097/01.brs.0000208128.49912.64