Insulin secretion and Ca2+ dynamics in β-cells are regulated by PERK (EIF2AK3) in concert with calcineurin

Rong Wang, Barbara Claire McGrath, Richard F. Kopp, Michael W. Roe, Xin Tang, Gong Chen, Douglas R. Cavener

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) (EIF2AK3) is essential for normal development and function of the insulin-secreting β-cell. Although genetic ablation of PERK in β-cells results in permanent neonatal diabetes in humans and mice, the underlying mechanisms remain unclear. Here, we used a newly developed and highly specific inhibitor of PERK to determine the immediate effects of acute ablation of PERK activity. We found that inhibition of PERK in human and rodent β-cells causes a rapid inhibition of secretagogue-stimulated subcellular Ca2+ signaling and insulin secretion. These dysfunctions stem from alterations in storeoperated Ca2+ entry and sarcoplasmic endoplasmic reticulum Ca 2+-ATPase activity. We also found that PERK regulates calcineurin, and pharmacological inhibition of calcineurin results in similar defects on stimulus-secretion coupling. Our findings suggest that interplay between calcineurin and PERK regulates β-cell Ca2+ signaling and insulin secretion, and that loss of this interaction may have profound implications in insulin secretion defects associated with diabetes.

Original languageEnglish (US)
Pages (from-to)33824-33836
Number of pages13
JournalJournal of Biological Chemistry
Volume288
Issue number47
DOIs
StatePublished - Nov 22 2013

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Calcineurin
Endoplasmic Reticulum
Phosphotransferases
Insulin
Medical problems
Ablation
Cell signaling
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Defects
Insulin-Secreting Cells
Protein Kinases
Adenosine Triphosphatases
Rodentia
Pharmacology

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Insulin secretion and Ca2+ dynamics in β-cells are regulated by PERK (EIF2AK3) in concert with calcineurin",
abstract = "Protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) (EIF2AK3) is essential for normal development and function of the insulin-secreting β-cell. Although genetic ablation of PERK in β-cells results in permanent neonatal diabetes in humans and mice, the underlying mechanisms remain unclear. Here, we used a newly developed and highly specific inhibitor of PERK to determine the immediate effects of acute ablation of PERK activity. We found that inhibition of PERK in human and rodent β-cells causes a rapid inhibition of secretagogue-stimulated subcellular Ca2+ signaling and insulin secretion. These dysfunctions stem from alterations in storeoperated Ca2+ entry and sarcoplasmic endoplasmic reticulum Ca 2+-ATPase activity. We also found that PERK regulates calcineurin, and pharmacological inhibition of calcineurin results in similar defects on stimulus-secretion coupling. Our findings suggest that interplay between calcineurin and PERK regulates β-cell Ca2+ signaling and insulin secretion, and that loss of this interaction may have profound implications in insulin secretion defects associated with diabetes.",
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Insulin secretion and Ca2+ dynamics in β-cells are regulated by PERK (EIF2AK3) in concert with calcineurin. / Wang, Rong; McGrath, Barbara Claire; Kopp, Richard F.; Roe, Michael W.; Tang, Xin; Chen, Gong; Cavener, Douglas R.

In: Journal of Biological Chemistry, Vol. 288, No. 47, 22.11.2013, p. 33824-33836.

Research output: Contribution to journalArticle

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AU - Tang, Xin

AU - Chen, Gong

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