Integrated microdevice for single-neuron in vivo electroporation

Ming Da Zhou, Yingwei Mao, Siyang Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report an integrated microdevice that can perform high spatial resolution, single-neuron level electroporation in deep brain. The microdevice was characterized with in vitro electroporation with MDA-MB-231 cells and cell membrane non-permeable dyes. In vivo electroporation with a plasmid that encodes the green fluorescent protein (GFP) as a reporter gene was successfully demonstrated at hippocampus of mouse brain, and single neuron that expresses green fluorescent protein was observed.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages345-347
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Neurons
Brain
Proteins
Cell membranes
Dyes
Genes

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Zhou, M. D., Mao, Y., & Zheng, S. (2014). Integrated microdevice for single-neuron in vivo electroporation. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 345-347). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.
Zhou, Ming Da ; Mao, Yingwei ; Zheng, Siyang. / Integrated microdevice for single-neuron in vivo electroporation. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 345-347 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
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title = "Integrated microdevice for single-neuron in vivo electroporation",
abstract = "We report an integrated microdevice that can perform high spatial resolution, single-neuron level electroporation in deep brain. The microdevice was characterized with in vitro electroporation with MDA-MB-231 cells and cell membrane non-permeable dyes. In vivo electroporation with a plasmid that encodes the green fluorescent protein (GFP) as a reporter gene was successfully demonstrated at hippocampus of mouse brain, and single neuron that expresses green fluorescent protein was observed.",
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Zhou, MD, Mao, Y & Zheng, S 2014, Integrated microdevice for single-neuron in vivo electroporation. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 345-347, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.

Integrated microdevice for single-neuron in vivo electroporation. / Zhou, Ming Da; Mao, Yingwei; Zheng, Siyang.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 345-347 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Zhou MD, Mao Y, Zheng S. Integrated microdevice for single-neuron in vivo electroporation. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 345-347. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).