Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes

Daguan Nong, Zachary K. Haviland, Kate Vasquez Kuntz, Ming Tien, Charles T. Anderson, William O. Hancock

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations

Abstract

We describe a multimodal microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines interference reflection microscopy (IRM) with multi-wavelength total internal reflectance fluorescence microscopy (TIRFM). The microscope can localize quantum dots with a precision of 2.8 nm at 100 frames/s, and was used to image the dynamics of the cellulase, Cel7a interacting with surface-immobilized cellulose. The instrument, which was built with off-the-shelf components and is controlled by custom software, is suitable for tracking other degradative enzymes such as collagenases, as well as motor proteins moving along immobilized tracks.

Original languageEnglish (US)
Pages (from-to)3253-3264
Number of pages12
JournalBiomedical Optics Express
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2021

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes'. Together they form a unique fingerprint.

Cite this