Integrated PCR and thermal melt analysis on a microfluidic chip

Deborah Boles, Michele Stone, Esther Huang, Ming Wu, Roger Dettloff, Aaron Rulison, Javier Farinas, Ivor Knight

Research output: Contribution to conferencePaperpeer-review

Abstract

We have modified and tested the microfluidic LabChip® system [1] to integrate thermal melt analysis and PCR in a continuous flow format utilizing reaction volumes of several nanoliters. Results demonstrate high resolution thermal melt analysis at ramp rates up to 1°C/s. To our knowledge, this is the first demonstration of a continuous flow system integrating PCR and thermal melt analysis.

Original languageEnglish (US)
Pages1444-1446
Number of pages3
StatePublished - Jan 1 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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